Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices

Yoon Ji Kwon; Sojeong Pak; Sunggu Yang; Sungchil Yang

doi:10.1016/j.xpro.2022.102030

Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices

Yoon Ji Kwon
, Sojeong Pak
, Sunggu Yang^*
, Sungchil Yang^*

^*Corresponding author for this work

Department of Neuroscience

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Longitudinal synaptic connections between dentate gyrus (DG) granule neurons in the hippocampus have been found to be correlated with increased anxiety. Here, we present a protocol to assess synaptic connectivity and plasticity in the longitudinal DG network. We detail the steps for (1) obtaining acute mouse hippocampal slices that contain longitudinal DG-DG connections, (2) measuring excitatory postsynaptic potentials using whole-cell patch clamp recording combined with two-photon microscopy and glutamate uncaging, and (3) assessing synaptic plasticity using extracellular field recording.

For complete details on the use and execution of this protocol, please refer to Pak et al. (2022).¹

Original language	English
Article number	102030
Journal	STAR Protocols
Volume	4
Issue number	1
Online published	11 Jan 2023
DOIs	https://doi.org/10.1016/j.xpro.2022.102030
Publication status	Published - 17 Mar 2023

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This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/

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Hearing Loss-induced Neural Plasticity and its Mechanism in the Temporal Lobe
PAK, S. (Author), YANG, S. (Supervisor), 27 Apr 2023
Student thesis: Doctoral Thesis

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title = "Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices",

abstract = "Longitudinal synaptic connections between dentate gyrus (DG) granule neurons in the hippocampus have been found to be correlated with increased anxiety. Here, we present a protocol to assess synaptic connectivity and plasticity in the longitudinal DG network. We detail the steps for (1) obtaining acute mouse hippocampal slices that contain longitudinal DG-DG connections, (2) measuring excitatory postsynaptic potentials using whole-cell patch clamp recording combined with two-photon microscopy and glutamate uncaging, and (3) assessing synaptic plasticity using extracellular field recording. For complete details on the use and execution of this protocol, please refer to Pak et al. (2022).1",

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AU - Kwon, Yoon Ji

AU - Pak, Sojeong

AU - Yang, Sunggu

AU - Yang, Sungchil

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Longitudinal synaptic connections between dentate gyrus (DG) granule neurons in the hippocampus have been found to be correlated with increased anxiety. Here, we present a protocol to assess synaptic connectivity and plasticity in the longitudinal DG network. We detail the steps for (1) obtaining acute mouse hippocampal slices that contain longitudinal DG-DG connections, (2) measuring excitatory postsynaptic potentials using whole-cell patch clamp recording combined with two-photon microscopy and glutamate uncaging, and (3) assessing synaptic plasticity using extracellular field recording. For complete details on the use and execution of this protocol, please refer to Pak et al. (2022).1

AB - Longitudinal synaptic connections between dentate gyrus (DG) granule neurons in the hippocampus have been found to be correlated with increased anxiety. Here, we present a protocol to assess synaptic connectivity and plasticity in the longitudinal DG network. We detail the steps for (1) obtaining acute mouse hippocampal slices that contain longitudinal DG-DG connections, (2) measuring excitatory postsynaptic potentials using whole-cell patch clamp recording combined with two-photon microscopy and glutamate uncaging, and (3) assessing synaptic plasticity using extracellular field recording. For complete details on the use and execution of this protocol, please refer to Pak et al. (2022).1

U2 - 10.1016/j.xpro.2022.102030

DO - 10.1016/j.xpro.2022.102030

M3 - RGC 21 - Publication in refereed journal

C2 - 36633949

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VL - 4

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 102030

ER -

Electrophysiological measurements of synaptic connectivity and plasticity in the longitudinal dentate gyrus network from mouse hippocampal slices

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Hearing Loss-induced Neural Plasticity and its Mechanism in the Temporal Lobe

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