Electronic concentration of negatively-charged molecules on a microfabricated biochip

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Monika Syrzycka
  • Michael Sjoerdsma
  • M. Parameswaran
  • Marek Syrzycki
  • Carol A. Koch
  • Raj S. Utkhede

Detail(s)

Original languageEnglish
Pages (from-to)1-14
Journal / PublicationAnalytica Chimica Acta
Volume484
Issue number1
Publication statusPublished - 7 May 2003
Externally publishedYes

Abstract

Various conditions leading to successful electronic concentration of negatively-charged molecules, notably oligonucleotides, on a microfabricated biochip have been studied. Tests on two chip designs have been performed. In the first generation biochip, which consisted of 12 electrodes 5mm apart, fluorescent molecules were attracted to a positively-biased electrode leading to electronic concentration. In the second generation biochip, which consisted of eight electrodes 250μm apart, much faster concentration rates were observed due to reduction in electrode spacing. These results are significant to design a pathogen detection biochip based on DNA hybridization assisted by electronic concentration. © 2003 Elsevier Science B.V. All rights reserved.

Research Area(s)

  • Biochip, Electronic concentration, Microarray, Microfabrication, Oligonucleotide

Bibliographic Note

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Citation Format(s)

Electronic concentration of negatively-charged molecules on a microfabricated biochip. / Syrzycka, Monika; Sjoerdsma, Michael; Li, Paul C.H.; Parameswaran, M.; Syrzycki, Marek; Koch, Carol A.; Utkhede, Raj S.

In: Analytica Chimica Acta, Vol. 484, No. 1, 07.05.2003, p. 1-14.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review