Abstract
Various conditions leading to successful electronic concentration of negatively-charged molecules, notably oligonucleotides, on a microfabricated biochip have been studied. Tests on two chip designs have been performed. In the first generation biochip, which consisted of 12 electrodes 5mm apart, fluorescent molecules were attracted to a positively-biased electrode leading to electronic concentration. In the second generation biochip, which consisted of eight electrodes 250μm apart, much faster concentration rates were observed due to reduction in electrode spacing. These results are significant to design a pathogen detection biochip based on DNA hybridization assisted by electronic concentration. © 2003 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 1-14 |
| Journal | Analytica Chimica Acta |
| Volume | 484 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 7 May 2003 |
| Externally published | Yes |
Bibliographical note
Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].Research Keywords
- Biochip
- Electronic concentration
- Microarray
- Microfabrication
- Oligonucleotide
