Effects-Directed Analysis of Dissolved Organic Compounds in Oil Sands Process-Affected Water

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

100 Scopus Citations
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Author(s)

  • Garrett D. Morandi
  • Steve B. Wiseman
  • Alberto Pereira
  • Rishikesh Mankidy
  • Ian G. M. Gault
  • And 2 others
  • Jonathan W. Martin
  • John P. Giesy

Detail(s)

Original languageEnglish
Pages (from-to)12395-12404
Journal / PublicationEnvironmental Science and Technology
Volume49
Issue number20
Publication statusPublished - 20 Oct 2015

Abstract

Acute toxicity of oil sands process-affected water (OSPW) is caused by its complex mixture of bitumen-derived organics, but the specific chemical classes that are most toxic have not been demonstrated. Here, effects-directed analysis was used to determine the most acutely toxic chemical classes in OSPW collected from the world's first oil sands end-pit lake. Three sequential rounds of fractionation, chemical analysis (ultrahigh resolution mass spectrometry), and acute toxicity testing (96 h fathead minnow embryo lethality and 15 min Microtox bioassay) were conducted. Following primary fractionation, toxicity was primarily attributable to the neutral extractable fraction (F1-NE), containing 27% of original organics mass. In secondary fractionation, F1-NE was subfractionated by alkaline water washing, and toxicity was primarily isolated to the ionizable fraction (F2-NE2), containing 18.5% of the original organic mass. In the final round, chromatographic subfractionation of F2-NE2 resulted in two toxic fractions, with the most potent (F3-NE2a, 11% of original organic mass) containing predominantly naphthenic acids (O2 -). The less-toxic fraction (F3-NE2b, 8% of original organic mass) contained predominantly nonacid species (O+, O2 +, SO+, NO+). Evidence supports naphthenic acids as among the most acutely toxic chemical classes in OSPW, but nonacidic species also contribute to acute toxicity of OSPW.

Citation Format(s)

Effects-Directed Analysis of Dissolved Organic Compounds in Oil Sands Process-Affected Water. / Morandi, Garrett D.; Wiseman, Steve B.; Pereira, Alberto; Mankidy, Rishikesh; Gault, Ian G. M.; Martin, Jonathan W.; Giesy, John P.

In: Environmental Science and Technology, Vol. 49, No. 20, 20.10.2015, p. 12395-12404.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review