Effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

9 Scopus Citations
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Author(s)

  • Zhi Wang
  • Quan Qing
  • Xi Chen
  • Cheng-Jun Liu
  • Jing-Cong Luo
  • Ting-Wu Qin

Detail(s)

Original languageEnglish
Pages (from-to)223-227
Journal / PublicationApplied Surface Science
Volume388
Issue numberPart A
Online published23 Jan 2016
Publication statusPublished - 1 Dec 2016
Externally publishedYes

Abstract

The purpose of this study was to investigate the effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs. Tenocytes were obtained from tail tendons of rats. Polydimethylsiloxane (PDMS) was used to fabricate three types of scaffolds with varying surface morphological characteristics, i.e., smooth, micro-grooved, and porous surfaces, respectively. The tenocytes were seeded on the surfaces of the scaffolds to form tenocyte-scaffold constructs. The constructs were cryopreserved in a vitreous cryoprotectant (CPA) with a multi-step protocol. The cell adhesion to scaffolds was observed with electronic scanning microscopy (SEM). The elongation index of the living tenocytes and ratio of live/dead cell number were examined based on a live/dead dual fluorescent staining technique, and the survival rate of tenocytes was studied with flow cytometry (FC). The results showed the shapes of tenocytes varied between the different groups: flat or polygonal (on smooth surface), spindle (on micro-grooved surface), and spindle or ellipse (on porous surface). After thawing, the porous surface got the most living tenocytes and a higher survival rate, suggesting its potential application for vitreous cryopreservation of engineered tendon constructs.

Research Area(s)

  • Adhesion, Scaffold, Surface morphology, Survival rate, Tenocyte, Vitreous cryopreservation

Citation Format(s)

Effects of scaffold surface morphology on cell adhesion and survival rate in vitreous cryopreservation of tenocyte-scaffold constructs. / Wang, Zhi; Qing, Quan; Chen, Xi; Liu, Cheng-Jun; Luo, Jing-Cong; Hu, Jin-Lian; Qin, Ting-Wu.

In: Applied Surface Science, Vol. 388, No. Part A, 01.12.2016, p. 223-227.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal