TY - JOUR
T1 - Effects of carbon dioxide concentration and swine wastewater on the cultivation of Chlorella vulgaris FSP-E and bioethanol production from microalgae biomass
AU - Condor, Billriz E.
AU - de Luna, Mark Daniel G.
AU - Lacson, Carl Francis Z.
AU - Acebu, Paula Isabel G.
AU - Abarca, Ralf Ruffel M.
AU - Nagarajan, Dillirani
AU - Lee, Duu-Jong
AU - Chang, Jo-Shu
PY - 2024/9/1
Y1 - 2024/9/1
N2 - The study investigated the potential of wastewater-derived microalgae as a cost-effective feedstock for bioethanol production. The influence of CO2 loading, use of swine wastewater as a nutrient source, effect of micronutrients in the cultivation media, and scale-up of Chlorella vulgaris FSP-E was conducted. Results showed that 2% CO2, 25% swine wastewater, 500 mg/L sodium nitrate, 40 mg/L K2HPO4, 75 mg/L MgSO4, and 6 mg/L citric acid were optimal for maximal biomass production, nitrate removal, and carbohydrate accumulation in BG-11 medium. The maximum biomass and carbohydrate content obtained in this study were 35-fold and 60%, respectively. Scaling up to 5 L volume was challenging due to illumination, aeration, and mixing issues. The obtained carbohydrate-rich biomass was subjected to mild sulfuric acid hydrolysis and subsequently used for bioethanol fermentation. The hydrolysate was composed solely of glucose and galactose and was converted into bioethanol within 8 h by Saccharomyces cerevisiae with an ethanol yield of 0.22 ± 0.01 g ethanol per g biomass. Further exploration of effective lighting and mixing strategies are recommended to realize economically viable microalgal biofuels. © 2024 Elsevier Ltd.
AB - The study investigated the potential of wastewater-derived microalgae as a cost-effective feedstock for bioethanol production. The influence of CO2 loading, use of swine wastewater as a nutrient source, effect of micronutrients in the cultivation media, and scale-up of Chlorella vulgaris FSP-E was conducted. Results showed that 2% CO2, 25% swine wastewater, 500 mg/L sodium nitrate, 40 mg/L K2HPO4, 75 mg/L MgSO4, and 6 mg/L citric acid were optimal for maximal biomass production, nitrate removal, and carbohydrate accumulation in BG-11 medium. The maximum biomass and carbohydrate content obtained in this study were 35-fold and 60%, respectively. Scaling up to 5 L volume was challenging due to illumination, aeration, and mixing issues. The obtained carbohydrate-rich biomass was subjected to mild sulfuric acid hydrolysis and subsequently used for bioethanol fermentation. The hydrolysate was composed solely of glucose and galactose and was converted into bioethanol within 8 h by Saccharomyces cerevisiae with an ethanol yield of 0.22 ± 0.01 g ethanol per g biomass. Further exploration of effective lighting and mixing strategies are recommended to realize economically viable microalgal biofuels. © 2024 Elsevier Ltd.
KW - Biofuels
KW - Carbohydrate accumulation
KW - Carbon capture
KW - Fermentation
KW - Photobioreactors
KW - Wastewater treatment
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U2 - 10.1016/j.apenergy.2024.123617
DO - 10.1016/j.apenergy.2024.123617
M3 - RGC 21 - Publication in refereed journal
SN - 0306-2619
VL - 369
JO - Applied Energy
JF - Applied Energy
M1 - 123617
ER -
