Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

46 Scopus Citations
View graph of relations

Author(s)

  • Ling Ding
  • Margaret B. Murphy
  • Yan Xu
  • Leo W.Y. Yeung
  • Jingxian Wang
  • Bingsheng Zhou
  • Paul K.S. Lam
  • Rudolf S.S. Wu
  • John P. Giesy

Detail(s)

Original languageEnglish
Pages (from-to)764-772
Journal / PublicationEnvironmental Toxicology and Chemistry
Volume26
Issue number4
Publication statusPublished - Apr 2007

Abstract

Brominated flame retardants (BFRs) and brominated dioxins are emerging persistent organic pollutants that are ubiquitous in the environment and can be accumulated by wildlife and humans. These chemicals can disturb endocrine function. Recent studies have demonstrated that one of the mechanisms of endocrine disruption by chemicals is modulation of steroidogenic gene expression or enzyme activities. In this study, an in vitro assay based on the H295R human adrenocortical carcinoma cell line, which possesses most key genes or enzymes involved in steroidogenesis, was used to examine the effects of five bromophenols, two polybrominated biphenyls (PBBs 77 and 169), 2,3,7,8-tetrabromodibenzo-p-dioxin, and 2,3,7,8-tetrabromodibenzofuran on the expression of 10 key steroidogenic genes. The H295R cells were exposed to various BFR concentrations for 48 h, and the expression of specific genes - cytochrome P450 (CYP11A, CYP11B2, CYP17, CYP19, and CYP21), 3β- hydroxysteroid dehydrogenase (3βHSD2), 17β-hydroxysteroid dehydrogenase (17βHSD1 and 17βHSD4), steroidogenic acute regulatory protein (StAR), and 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) - was quantitatively measured using real-time polymerase chain reaction. Cell viability was not affected at the doses tested. Most of the genes were either up- or down-regulated, to some extent, by BFR exposure. Among the genes tested, 3βHSD2 was the most markedly up-regulated, with a range of magnitude from 1.6- to 20-fold. The results demonstrate that bromophenol, bromobiphenyls, and bromodibenzo-p-dioxin/furan are able to modulate steroidogenic gene expression, which may lead to endocrine disruption. © 2007 SETAC.

Research Area(s)

  • Brominated flame retardants, Bromodioxins, Gene expression, H295R, Steroidogenesis

Citation Format(s)

Effects of brominated flame retardants and brominated dioxins on steroidogenesis in H295R human adrenocortical carcinoma cell line. / Ding, Ling; Murphy, Margaret B.; He, Yuhe et al.
In: Environmental Toxicology and Chemistry, Vol. 26, No. 4, 04.2007, p. 764-772.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review