TY - JOUR
T1 - Effects of Bis(imino)acenaphthene (Bian)-Derived Ligands on the Cytotoxicity, DNA Interactions, and Redox Activity of Palladium(II) Bipyridine Complexes
AU - Komlyagina, Veronika I.
AU - Romashev, Nikolai F.
AU - Besprozvannykh, Victoria K.
AU - Arakelyan, Jemma
AU - Wu, Chengnan
AU - Chubarov, Alexey S.
AU - Bakaev, Ivan V.
AU - Soh, Yee Kiat
AU - Abramov, Pavel A.
AU - Cheung, Kin Leung
AU - Kompan'kov, Nikolai B.
AU - Ryadun, Aleksey A.
AU - Babak, Maria V.
AU - Gushchin, Artem L.
PY - 2023/7/24
Y1 - 2023/7/24
N2 - A series of heteroleptic bipyridine Pd(II) complexes based on 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene (dpp-Bian) or 1,2-bis[(2,4,6-trimethylphenyl)imino]acenaphthene (tmp-Bian) were prepared. All complexes were fully characterized by spectrochemical methods, and their crystal structures were confirmed by X-ray diffraction analysis. The 72 h stability of heteroleptic bipyridine Pd(II) complexes with Bian ligands under physiological conditions was investigated using 1H NMR spectroscopy. The anticancer activity of all complexes was assessed in a panel of cancer cell lines in comparison with uncoordinated ligands and clinically used drugs cisplatin and doxorubicin. The ability of the complexes to bind DNA was investigated using several methods, including EtBr replacement assay, density functional theory calculations, circular dichroism spectroscopy, DNA gel electrophoresis, and TUNEL assay. The electrochemical activity of all complexes and the uncoordinated ligands was studied using cyclic voltammetry, and reactive oxygen species production in cancer cells was investigated using confocal microscopy. Heteroleptic bipyridine PdII-Bian complexes were cytotoxic in a low micromolar concentration range and showed some selectivity toward cancer cells in comparison with noncancerous MRC-5 lung fibroblasts. © 2023 American Chemical Society.
AB - A series of heteroleptic bipyridine Pd(II) complexes based on 1,2-bis[(2,6-diisopropylphenyl)imino]acenaphthene (dpp-Bian) or 1,2-bis[(2,4,6-trimethylphenyl)imino]acenaphthene (tmp-Bian) were prepared. All complexes were fully characterized by spectrochemical methods, and their crystal structures were confirmed by X-ray diffraction analysis. The 72 h stability of heteroleptic bipyridine Pd(II) complexes with Bian ligands under physiological conditions was investigated using 1H NMR spectroscopy. The anticancer activity of all complexes was assessed in a panel of cancer cell lines in comparison with uncoordinated ligands and clinically used drugs cisplatin and doxorubicin. The ability of the complexes to bind DNA was investigated using several methods, including EtBr replacement assay, density functional theory calculations, circular dichroism spectroscopy, DNA gel electrophoresis, and TUNEL assay. The electrochemical activity of all complexes and the uncoordinated ligands was studied using cyclic voltammetry, and reactive oxygen species production in cancer cells was investigated using confocal microscopy. Heteroleptic bipyridine PdII-Bian complexes were cytotoxic in a low micromolar concentration range and showed some selectivity toward cancer cells in comparison with noncancerous MRC-5 lung fibroblasts. © 2023 American Chemical Society.
KW - HETEROCYCLIC CARBENE COMPLEXES
KW - COPPER(II) COMPLEXES
KW - ACENAPHTHENE-1,2-DIIMINE LIGAND
KW - INSERTION POLYMERIZATION
KW - BULKY
KW - SUBSTITUENTS
KW - SPECTROSCOPY
KW - REDUCTION
KW - CISPLATIN
KW - APOPTOSIS
UR - http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=LinksAMR&SrcApp=PARTNER_APP&DestLinkType=FullRecord&DestApp=WOS&KeyUT=001022383400001
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85165546424&origin=recordpage
UR - http://www.scopus.com/inward/record.url?scp=85165546424&partnerID=8YFLogxK
U2 - 10.1021/acs.inorgchem.3c01172
DO - 10.1021/acs.inorgchem.3c01172
M3 - RGC 21 - Publication in refereed journal
SN - 0020-1669
VL - 62
SP - 11541
EP - 11553
JO - Inorganic Chemistry
JF - Inorganic Chemistry
IS - 29
ER -
