TY - JOUR
T1 - Effects of 17β-estradiol on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic trans-differentiation of osteoblasts
AU - Li, Xiang-Hui
AU - Zhang, Jin-Chao
AU - Liu, Chang-Zhen
AU - Sui, Sen-Fang
AU - Yang, Meng-Su
PY - 2006/9
Y1 - 2006/9
N2 - OBJECTIVE: To study the effects of 17 β-estradiol on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells(MSCs)and the adipocytic trans-differentiation of primary mouse osteoblasts(OBs). METHODS: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) was used to study the effects of 17 β-estradiol on the growth of MSCs and OBs, alkaline phosphatase(ALP)activity assay was used to study the effects of 17 β-estradiol on osteogenic differentiation of MSCs, and Oil Red O assay was used to study the effects of 17 β-estradiol on the adipogenic differentiation of MSCs and adipocytic trans-differentiation of OBs. RESULTS: 17 β-estradiol promoted the proliferation of primary mouse bone marrow stromal cells and primary mouse osteoblasts at the concentrations of 1 × 10-8, 1 × 10-7, 5 × 10-7, 1 × 10-6, 5 × 10-6 and 1 × 10 -5 mol·L-1(P <0.05). 17 β-estradiol promoted osteogenic differentiation and inhibited the adipogenic differentiation of MSCs at the concentrations of 5 × 10-7, 1 × 10 -6, 5 × 10-6 and 1 × 10-5 mol·L-1, but inhibited the osteogenic differentiation and promoted adipogenic differentiation of MSCs at the concentration of 1 × 10-7 mol·L-1 (P <0.05). 17 β-estradiol inhibited the adipocytic transdifferentiation of OBs at the concentrations of 1 × 10-8, 5 × 10-7, 1 × 10-6, 1 × 10-5 mol·L-1(P <0.05). CONCLUSION: 17 β-estradiol can modulate the differentiation of marrow stromal cells to cause a lineage shift toward the osteoblast and away from the adipocyte and inhibit the adipocytic trans-differentiation of osteoblasts.
AB - OBJECTIVE: To study the effects of 17 β-estradiol on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells(MSCs)and the adipocytic trans-differentiation of primary mouse osteoblasts(OBs). METHODS: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide(MTT) was used to study the effects of 17 β-estradiol on the growth of MSCs and OBs, alkaline phosphatase(ALP)activity assay was used to study the effects of 17 β-estradiol on osteogenic differentiation of MSCs, and Oil Red O assay was used to study the effects of 17 β-estradiol on the adipogenic differentiation of MSCs and adipocytic trans-differentiation of OBs. RESULTS: 17 β-estradiol promoted the proliferation of primary mouse bone marrow stromal cells and primary mouse osteoblasts at the concentrations of 1 × 10-8, 1 × 10-7, 5 × 10-7, 1 × 10-6, 5 × 10-6 and 1 × 10 -5 mol·L-1(P <0.05). 17 β-estradiol promoted osteogenic differentiation and inhibited the adipogenic differentiation of MSCs at the concentrations of 5 × 10-7, 1 × 10 -6, 5 × 10-6 and 1 × 10-5 mol·L-1, but inhibited the osteogenic differentiation and promoted adipogenic differentiation of MSCs at the concentration of 1 × 10-7 mol·L-1 (P <0.05). 17 β-estradiol inhibited the adipocytic transdifferentiation of OBs at the concentrations of 1 × 10-8, 5 × 10-7, 1 × 10-6, 1 × 10-5 mol·L-1(P <0.05). CONCLUSION: 17 β-estradiol can modulate the differentiation of marrow stromal cells to cause a lineage shift toward the osteoblast and away from the adipocyte and inhibit the adipocytic trans-differentiation of osteoblasts.
KW - 17β-estradiol
KW - Adipocytic trans-differentiation
KW - Adipogenic differentiation
KW - Bone marrow stromal cells
KW - Osteoblasts
KW - Osteogenic differentiation
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M3 - RGC 22 - Publication in policy or professional journal
SN - 1001-2494
VL - 41
SP - 1305
EP - 1309
JO - Chinese Pharmaceutical Journal
JF - Chinese Pharmaceutical Journal
IS - 17
ER -