Abstract
Abundant evidence now supports the idea that methionine deficiency has negative effects on chicken healthy especially in the aspect of cell cycle regulation and apoptosis. But, lacking of knowledge is the evaluation on metabolic organs. To test the effect of methionine deficiency on the kidney, we assessed the apoptosis and the cell cycle of kidney induced by methionine deficiency by the methods of TdT-mediated dUTP Nick-End Labeling (TUNEL), flow cytometry (FCM), quantitative real-time PCR (qRT-PCR) and Enzyme-Linked ImmunoSorbent Assay (ELISA) of chickens for 42 days of age. Our results showed that the number of the apoptotic cells was increased (p
| Original language | English |
|---|---|
| Pages (from-to) | 228-236 |
| Number of pages | 9 |
| Journal | Research in Veterinary Science |
| Volume | 135 |
| Online published | 16 Oct 2019 |
| DOIs | |
| Publication status | Published - Mar 2021 |
| Externally published | Yes |
Funding
The study was supported by the programme for Scientific Research Projects of China West Normal University (project no. 16B002). The authors would like to thank the co-workers of China West Normal University for their assistance in performing the experiment and the analysis.
Research Keywords
- Methionine deficiency
- Apoptosis
- Cell cycle
- Broilers