Effect of loop sequence and loop length on the intrinsic fluorescence of G-quadruplexes

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

30 Scopus Citations
View graph of relations

Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)3019-3021
Journal / PublicationBiochemistry
Volume52
Issue number18
Publication statusPublished - 7 May 2013
Externally publishedYes

Abstract

Guanine quadruplex structures (GQSs) exhibit unique spectroscopic features, including an inverse melting profile at 295 nm, distinctive circular dichroism features, and intrinsic fluorescence. Herein, we investigate effects of loop sequence and loop length on the intrinsic fluorescence of 13 DNA GQSs. We report label-free fluorescence enhancements upon intramolecular GQS formation of up to 16-fold and a shift in the emission maximum to the visible portion of the spectrum. Effects can be understood in the context of available nuclear magnetic resonance GQS structures. The intrinsic fluorescence of GQSs may be useful for nucleic acid studies and for the development of label-free detection methods. © 2013 American Chemical Society.

Citation Format(s)

Effect of loop sequence and loop length on the intrinsic fluorescence of G-quadruplexes. / Kwok, Chun Kit; Sherlock, Madeline E.; Bevilacqua, Philip C.

In: Biochemistry, Vol. 52, No. 18, 07.05.2013, p. 3019-3021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal