Effect of lanthanum (III) on cytosolic free calcium in isolated rabbit mature osteoclasts

Jinchao Zhang; Tianlan Zhang; Shanjin Xu; Kui Wang; Shifeng Yu; Mengsu Yang

doi:10.1115/1.1897745

Effect of lanthanum (III) on cytosolic free calcium in isolated rabbit mature osteoclasts

Jinchao Zhang, Tianlan Zhang, Shanjin Xu, Kui Wang, Shifeng Yu, Mengsu Yang

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

14 Citations (Scopus)

Abstract

The effect of lanthanum (III) (La³⁺) on cytosolic free calcium ([Ca²⁺]_i) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La³⁺ does not alter basal [Ca²⁺]; levels and cell spread area at the concentration of 1.00 × 10^-8 mol·L^-1. However, La³⁺ at higher concentrations (1.00 × l0^-5 and 1.00 × 10^-7 mol·L^-1) decreases [Ca²⁺]_i levels and cell spread area, and greater decreases are observed for the higher concentrations of La³⁺. Since [Ca²⁺]_i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La³⁺ inhibit bone resorption by decreasing [Ca²⁺]; in rabbit mature osteoclasts.

Original language	English
Pages (from-to)	580-583
Journal	Journal of Rare Earths
Volume	23
Issue number	5
DOIs	https://doi.org/10.1115/1.1897745
Publication status	Published - Oct 2005

Research Keywords

Cytosolic free calcium
Fluo-3/AM
Lanthanum (III)
Osteoclasts
Rare earths

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title = "Effect of lanthanum (III) on cytosolic free calcium in isolated rabbit mature osteoclasts",

abstract = "The effect of lanthanum (III) (La3+) on cytosolic free calcium ([Ca2+]i) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La3+ does not alter basal [Ca2+]; levels and cell spread area at the concentration of 1.00 × 10-8 mol·L-1. However, La3+ at higher concentrations (1.00 × l0-5 and 1.00 × 10-7 mol·L-1) decreases [Ca2+]i levels and cell spread area, and greater decreases are observed for the higher concentrations of La3+. Since [Ca2+]i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La3+ inhibit bone resorption by decreasing [Ca2+]; in rabbit mature osteoclasts.",

keywords = "Cytosolic free calcium, Fluo-3/AM, Lanthanum (III), Osteoclasts, Rare earths",

author = "Jinchao Zhang and Tianlan Zhang and Shanjin Xu and Kui Wang and Shifeng Yu and Mengsu Yang",

year = "2005",

month = oct,

doi = "10.1115/1.1897745",

language = "English",

volume = "23",

pages = "580--583",

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TY - JOUR

T1 - Effect of lanthanum (III) on cytosolic free calcium in isolated rabbit mature osteoclasts

AU - Zhang, Jinchao

AU - Zhang, Tianlan

AU - Xu, Shanjin

AU - Wang, Kui

AU - Yu, Shifeng

AU - Yang, Mengsu

PY - 2005/10

Y1 - 2005/10

N2 - The effect of lanthanum (III) (La3+) on cytosolic free calcium ([Ca2+]i) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La3+ does not alter basal [Ca2+]; levels and cell spread area at the concentration of 1.00 × 10-8 mol·L-1. However, La3+ at higher concentrations (1.00 × l0-5 and 1.00 × 10-7 mol·L-1) decreases [Ca2+]i levels and cell spread area, and greater decreases are observed for the higher concentrations of La3+. Since [Ca2+]i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La3+ inhibit bone resorption by decreasing [Ca2+]; in rabbit mature osteoclasts.

AB - The effect of lanthanum (III) (La3+) on cytosolic free calcium ([Ca2+]i) in isolated rabbit mature osteoclasts was studied with the employment of fluo-3/AM as an intracellular calcium-sensitive fluorescent probe by using a confocal laser scanning microscope. La3+ does not alter basal [Ca2+]; levels and cell spread area at the concentration of 1.00 × 10-8 mol·L-1. However, La3+ at higher concentrations (1.00 × l0-5 and 1.00 × 10-7 mol·L-1) decreases [Ca2+]i levels and cell spread area, and greater decreases are observed for the higher concentrations of La3+. Since [Ca2+]i affects cytoskeleton and the adhesion properties of osteoclasts, our results seem to suggest that La3+ inhibit bone resorption by decreasing [Ca2+]; in rabbit mature osteoclasts.

KW - Cytosolic free calcium

KW - Fluo-3/AM

KW - Lanthanum (III)

KW - Osteoclasts

KW - Rare earths

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U2 - 10.1115/1.1897745

DO - 10.1115/1.1897745

M3 - RGC 21 - Publication in refereed journal

SN - 1002-0721

VL - 23

SP - 580

EP - 583

JO - Journal of Rare Earths

JF - Journal of Rare Earths

IS - 5

ER -

Effect of lanthanum (III) on cytosolic free calcium in isolated rabbit mature osteoclasts

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