Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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Detail(s)
Original language | English |
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Pages (from-to) | 57-68 |
Journal / Publication | Magnetic Resonance in Medicine |
Volume | 92 |
Issue number | 1 |
Online published | 2 Feb 2024 |
Publication status | Published - Jul 2024 |
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DOI | DOI |
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Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85184268338&origin=recordpage |
Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(1eb6d509-412c-474c-806d-9cb5f6dc89f4).html |
Abstract
Purpose: To investigate the effect of inhaled oxygen level on dynamic glucose enhanced (DGE) MRI in mouse brain tissue and CSF at 3 T.
Methods: DGE data of brain tissue and CSF from mice under normoxia or hyperoxia were acquired in independent and interleaved experiments using on-resonance variable delay multi-pulse (onVDMP) MRI. A bolus of 0.15 mL filtered 50% D-glucose was injected through the tail vein over 1 min during DGE acquisition. MRS was acquired before and after DGE experiments to confirm the presence of D-glucose.
Results: A significantly higher DGE effect under normoxia than under hyperoxia was observed in brain tissue (p = 0.0001 and p = 0.0002 for independent and interleaved experiments, respectively), but not in CSF (p > 0.3). This difference is attributed to the increased baseline MR tissue signal under hyperoxia induced by a shortened T1 and an increased BOLD effect. When switching from hyperoxia to normoxia without glucose injection, a signal change of ∼3.0% was found in brain tissue and a signal change of ∼1.5% was found in CSF.
Conclusions: DGE signal was significantly lower under hyperoxia than that under normoxia in brain tissue, but not in CSF. The reason is that DGE effect size of brain tissue is affected by the baseline signal, which could be influenced by T1 change and BOLD effect. Therefore, DGE experiments in which the oxygenation level is changed from baseline need to be interpreted carefully. © 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.
Methods: DGE data of brain tissue and CSF from mice under normoxia or hyperoxia were acquired in independent and interleaved experiments using on-resonance variable delay multi-pulse (onVDMP) MRI. A bolus of 0.15 mL filtered 50% D-glucose was injected through the tail vein over 1 min during DGE acquisition. MRS was acquired before and after DGE experiments to confirm the presence of D-glucose.
Results: A significantly higher DGE effect under normoxia than under hyperoxia was observed in brain tissue (p = 0.0001 and p = 0.0002 for independent and interleaved experiments, respectively), but not in CSF (p > 0.3). This difference is attributed to the increased baseline MR tissue signal under hyperoxia induced by a shortened T1 and an increased BOLD effect. When switching from hyperoxia to normoxia without glucose injection, a signal change of ∼3.0% was found in brain tissue and a signal change of ∼1.5% was found in CSF.
Conclusions: DGE signal was significantly lower under hyperoxia than that under normoxia in brain tissue, but not in CSF. The reason is that DGE effect size of brain tissue is affected by the baseline signal, which could be influenced by T1 change and BOLD effect. Therefore, DGE experiments in which the oxygenation level is changed from baseline need to be interpreted carefully. © 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.
Research Area(s)
- blood oxygenation level-dependent (BOLD), brain tissue, cerebrospinal fluid (CSF), dynamic glucose enhanced (DGE) MRI, oxygen, T1 relaxation
Citation Format(s)
Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain. / Huang, Jianpan; Chen, Zilin; van Zijl, Peter C. M. et al.
In: Magnetic Resonance in Medicine, Vol. 92, No. 1, 07.2024, p. 57-68.
In: Magnetic Resonance in Medicine, Vol. 92, No. 1, 07.2024, p. 57-68.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
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