Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain

Jianpan Huang; Zilin Chen; Peter C. M. van Zijl; Lok Hin Law; Rohith Saai Pemmasani Prabakaran; Se Weon Park; Jiadi Xu; Kannie W. Y. Chan

doi:10.1002/mrm.30035

Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain

Jianpan Huang, Zilin Chen, Peter C. M. van Zijl, Lok Hin Law, Rohith Saai Pemmasani Prabakaran, Se Weon Park, Jiadi Xu, Kannie W. Y. Chan^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

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Abstract

Purpose:  To investigate the effect of inhaled oxygen level on dynamic glucose enhanced (DGE) MRI in mouse brain tissue and CSF at 3 T.

Methods:  DGE data of brain tissue and CSF from mice under normoxia or hyperoxia were acquired in independent and interleaved experiments using on-resonance variable delay multi-pulse (onVDMP) MRI. A bolus of 0.15 mL filtered 50% D-glucose was injected through the tail vein over 1 min during DGE acquisition. MRS was acquired before and after DGE experiments to confirm the presence of D-glucose.

Results:  A significantly higher DGE effect under normoxia than under hyperoxia was observed in brain tissue (p = 0.0001 and p = 0.0002 for independent and interleaved experiments, respectively), but not in CSF (p > 0.3). This difference is attributed to the increased baseline MR tissue signal under hyperoxia induced by a shortened T₁ and an increased BOLD effect. When switching from hyperoxia to normoxia without glucose injection, a signal change of ∼3.0% was found in brain tissue and a signal change of ∼1.5% was found in CSF.

Conclusions:  DGE signal was significantly lower under hyperoxia than that under normoxia in brain tissue, but not in CSF. The reason is that DGE effect size of brain tissue is affected by the baseline signal, which could be influenced by T₁ change and BOLD effect. Therefore, DGE experiments in which the oxygenation level is changed from baseline need to be interpreted carefully. © 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.

Original language	English
Pages (from-to)	57-68
Journal	Magnetic Resonance in Medicine
Volume	92
Issue number	1
Online published	2 Feb 2024
DOIs	https://doi.org/10.1002/mrm.30035
Publication status	Published - Jul 2024

Funding

This work was supported by the Research Grants Council (11102218, 11200422, RFS2223-1S02, PDFS2122-1S01, and C1134-20G); City University of Hong Kong (7005433, 7005626, 9610616, 9609307, and 9610560); National Natural Science Foundation of China (81871409); Tung Biomedical Sciences Centre; Hong Kong Centre for Cerebro-cardiovascular Health Engineering; The University of Hong Kong (109000487, 204610401 and 204610519); and National Institutes of Health (R01EB034978).

Research Keywords

blood oxygenation level-dependent (BOLD)
brain tissue
cerebrospinal fluid (CSF)
dynamic glucose enhanced (DGE) MRI
oxygen
T1 relaxation

Publisher's Copyright Statement

This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

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RGC-funded

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@article{1eb6d509412c474c806d9cb5f6dc89f4,

title = "Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain",

abstract = "Purpose: To investigate the effect of inhaled oxygen level on dynamic glucose enhanced (DGE) MRI in mouse brain tissue and CSF at 3 T.Methods: DGE data of brain tissue and CSF from mice under normoxia or hyperoxia were acquired in independent and interleaved experiments using on-resonance variable delay multi-pulse (onVDMP) MRI. A bolus of 0.15 mL filtered 50% D-glucose was injected through the tail vein over 1 min during DGE acquisition. MRS was acquired before and after DGE experiments to confirm the presence of D-glucose.Results: A significantly higher DGE effect under normoxia than under hyperoxia was observed in brain tissue (p = 0.0001 and p = 0.0002 for independent and interleaved experiments, respectively), but not in CSF (p > 0.3). This difference is attributed to the increased baseline MR tissue signal under hyperoxia induced by a shortened T1 and an increased BOLD effect. When switching from hyperoxia to normoxia without glucose injection, a signal change of ∼3.0% was found in brain tissue and a signal change of ∼1.5% was found in CSF.Conclusions: DGE signal was significantly lower under hyperoxia than that under normoxia in brain tissue, but not in CSF. The reason is that DGE effect size of brain tissue is affected by the baseline signal, which could be influenced by T1 change and BOLD effect. Therefore, DGE experiments in which the oxygenation level is changed from baseline need to be interpreted carefully. {\textcopyright} 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.",

keywords = "blood oxygenation level-dependent (BOLD), brain tissue, cerebrospinal fluid (CSF), dynamic glucose enhanced (DGE) MRI, oxygen, T1 relaxation",

author = "Jianpan Huang and Zilin Chen and {van Zijl}, {Peter C. M.} and Law, {Lok Hin} and {Pemmasani Prabakaran}, {Rohith Saai} and Park, {Se Weon} and Jiadi Xu and Chan, {Kannie W. Y.}",

year = "2024",

month = jul,

doi = "10.1002/mrm.30035",

language = "English",

volume = "92",

pages = "57--68",

journal = "Magnetic Resonance in Medicine",

issn = "0740-3194",

publisher = "John Wiley & Sons",

number = "1",

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TY - JOUR

T1 - Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain

AU - Huang, Jianpan

AU - Chen, Zilin

AU - van Zijl, Peter C. M.

AU - Law, Lok Hin

AU - Pemmasani Prabakaran, Rohith Saai

AU - Park, Se Weon

AU - Xu, Jiadi

AU - Chan, Kannie W. Y.

PY - 2024/7

Y1 - 2024/7

N2 - Purpose: To investigate the effect of inhaled oxygen level on dynamic glucose enhanced (DGE) MRI in mouse brain tissue and CSF at 3 T.Methods: DGE data of brain tissue and CSF from mice under normoxia or hyperoxia were acquired in independent and interleaved experiments using on-resonance variable delay multi-pulse (onVDMP) MRI. A bolus of 0.15 mL filtered 50% D-glucose was injected through the tail vein over 1 min during DGE acquisition. MRS was acquired before and after DGE experiments to confirm the presence of D-glucose.Results: A significantly higher DGE effect under normoxia than under hyperoxia was observed in brain tissue (p = 0.0001 and p = 0.0002 for independent and interleaved experiments, respectively), but not in CSF (p > 0.3). This difference is attributed to the increased baseline MR tissue signal under hyperoxia induced by a shortened T1 and an increased BOLD effect. When switching from hyperoxia to normoxia without glucose injection, a signal change of ∼3.0% was found in brain tissue and a signal change of ∼1.5% was found in CSF.Conclusions: DGE signal was significantly lower under hyperoxia than that under normoxia in brain tissue, but not in CSF. The reason is that DGE effect size of brain tissue is affected by the baseline signal, which could be influenced by T1 change and BOLD effect. Therefore, DGE experiments in which the oxygenation level is changed from baseline need to be interpreted carefully. © 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.

AB - Purpose: To investigate the effect of inhaled oxygen level on dynamic glucose enhanced (DGE) MRI in mouse brain tissue and CSF at 3 T.Methods: DGE data of brain tissue and CSF from mice under normoxia or hyperoxia were acquired in independent and interleaved experiments using on-resonance variable delay multi-pulse (onVDMP) MRI. A bolus of 0.15 mL filtered 50% D-glucose was injected through the tail vein over 1 min during DGE acquisition. MRS was acquired before and after DGE experiments to confirm the presence of D-glucose.Results: A significantly higher DGE effect under normoxia than under hyperoxia was observed in brain tissue (p = 0.0001 and p = 0.0002 for independent and interleaved experiments, respectively), but not in CSF (p > 0.3). This difference is attributed to the increased baseline MR tissue signal under hyperoxia induced by a shortened T1 and an increased BOLD effect. When switching from hyperoxia to normoxia without glucose injection, a signal change of ∼3.0% was found in brain tissue and a signal change of ∼1.5% was found in CSF.Conclusions: DGE signal was significantly lower under hyperoxia than that under normoxia in brain tissue, but not in CSF. The reason is that DGE effect size of brain tissue is affected by the baseline signal, which could be influenced by T1 change and BOLD effect. Therefore, DGE experiments in which the oxygenation level is changed from baseline need to be interpreted carefully. © 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.

KW - blood oxygenation level-dependent (BOLD)

KW - brain tissue

KW - cerebrospinal fluid (CSF)

KW - dynamic glucose enhanced (DGE) MRI

KW - oxygen

KW - T1 relaxation

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U2 - 10.1002/mrm.30035

DO - 10.1002/mrm.30035

M3 - RGC 21 - Publication in refereed journal

C2 - 38308151

SN - 0740-3194

VL - 92

SP - 57

EP - 68

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

IS - 1

ER -

Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain

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Effect of inhaled oxygen level on dynamic glucose-enhanced MRI in mouse brain

Abstract

Funding

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

Other Access Options

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Other Files and Links

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RFS: Non-invasive CEST MRI Detects Reduced CSF Clearance in the Brain for Early Diagnosis of Alzheimer’s Disease

GRF: Imaging Nose-to-brain Drug Delivery to Brain Tumors Using Multiple CEST MRI Contrast

CRF: A Novel Vaccination Strategy: Using a Microrobot Platform for DNA Vaccine Delivery and Antigen Presentation

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