TY - JOUR
T1 - Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts
AU - Li, Xiang-Hui
AU - Zhang, Jin-Chao
AU - Sui, Sen-Fang
AU - Yang, Meng-Su
PY - 2005/9
Y1 - 2005/9
N2 - Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. Results: Daidzin, genistin, and glycitin 1 × 10-8, 5 × 10-7, 1 × 10-6, 5 × 10-6, and 1 × 10-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5 × 10-7 mol/L and genistin 1 × 10-6 mol/L promoted the osteogenesis of MSC. Genistin 1 × 10-8, 5 × 10-7, 1 × 10 -6, 5 × 10-6, and 1 × 10-5 mol/L and glycitin 1 × 10-8, 1 × 10-6, and 1 × 10-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1 × 10-8, 5 × 10-7, 1 × 10-6, 5 × 10-6, and 1 × 10-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis. ©2005 CPS and SIMM.
AB - Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. Results: Daidzin, genistin, and glycitin 1 × 10-8, 5 × 10-7, 1 × 10-6, 5 × 10-6, and 1 × 10-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5 × 10-7 mol/L and genistin 1 × 10-6 mol/L promoted the osteogenesis of MSC. Genistin 1 × 10-8, 5 × 10-7, 1 × 10 -6, 5 × 10-6, and 1 × 10-5 mol/L and glycitin 1 × 10-8, 1 × 10-6, and 1 × 10-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1 × 10-8, 5 × 10-7, 1 × 10-6, 5 × 10-6, and 1 × 10-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis. ©2005 CPS and SIMM.
KW - Adipocytic transdifferentiation
KW - Adipogenic differentiation
KW - Bone marrow stromal cells
KW - Daidzin
KW - Genistin
KW - Glycitin
KW - Osteoblasts
KW - Osteogenic differentiation
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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-24744447970&origin=recordpage
U2 - 10.1111/j.1745-7254.2005.00161.x
DO - 10.1111/j.1745-7254.2005.00161.x
M3 - RGC 21 - Publication in refereed journal
C2 - 16115375
SN - 1671-4083
VL - 26
SP - 1081
EP - 1086
JO - Acta Pharmacologica Sinica
JF - Acta Pharmacologica Sinica
IS - 9
ER -