Effect of culture conditions on the kinetics of hydrogen production by photosynthetic bacteria in batch culture

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Pages (from-to)14004-14013
Journal / PublicationInternational Journal of Hydrogen Energy
Issue number21
Publication statusPublished - Oct 2011
Externally publishedYes


Biochemical kinetic characteristics of photo-fermentative hydrogen production were experimentally and numerically investigated to optimize the photo-fermentation hydrogen-producing process in this work. It is found that a maximum specific growth rate of 0.26 h-1 was achieved under the optimal conditions of illumination intensity 6000 lux, 30 °C culture temperature and pH 7.0 of culture medium. These experimental results also led to an empirical formula of the maximum specific microbial growth rate (μmax) as a function of illumination intensity, pH and temperature. With the empirical formula, the modified Monod equation along with the kinetic equations for biomass growth, glucose consumption and hydrogen production is then developed to simulate the photofermentation hydrogen-producing process. The modeling results are in good agreements with the experimental data, indicating that the developed kinetic models are able to objectively describe the characteristics of hydrogen production by PSB under different culture conditions. © 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

Research Area(s)

  • Biohydrogen production, Culture temperature, Illumination intensity, pH value, Photosynthetic bacteria, Reaction kinetics

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