Effect of characteristics on the behavior of cultured human umbilical vein endothelial cells (HUVEC) onto titanium oxide film surface fabricated by plasma immersion ion implantation and deposition (PIIID)

It is believed that covering of endothelial cells on artificial cardiovascular devices surface may overcome surface induced thrombosis occurrence because of non-thrombogenic properties of the nature endothelial cells in contacting with blood. Therefore, endothelialization of prosthesis surface has been regard as an important mean to prevent thrombogenicity. Our previous research results revealed that the optimum Ti-O film possessed excellent blood compatibility. In this paper, we purposefully synthesized Ti-O films onto Si(100) wafer using plasma immersion ion implantation and deposition (PIII-D) with different process parameters for investigating the tissue compatibility of films with endothelial cell. The characteristics of the Ti-O films were investigated using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Atom force microscopy (AFM). The biological behavior of endothelial cells on the film surface was studied by in vitro HUVEC culture investigation. A series of Ti-O films were synthesized by generating Ti plasma from a Ti cathode mounted on the metal vacuum-arc plasma source and incorporating with oxygen. The analysis results proved out that the films possessed different characterization such as crystal structure, composition, and surface morphology etc. Investigation in vitro of the cultured HUVEC on Ti-O film surface enunciated that the growth, proliferation behavior of endothelial cells on different film material surface was significant different. The adherence, growth, shapes, and proliferation of endothelial cells on Ti-O film with crystalline, low surface energy, and smoother surface was excellent.