@inbook{e94e2b23bb2a4234a170cb7f5d43e74b,
title = "Effect of Carbon Nanotubes on HepG2 Adhesion and Spreading",
abstract = "There are several in vitro cell models for studying the interactions of carbon nanotubes (CNTs) with biological systems. This chapter provides a detailed protocol for studying the effects of CNTs on cell adhesion and spreading. The protocol is a combination of methods of electron microscopy, cell biology, and molecular biology, focusing on the detection of the cell morphology and cellular responses related to cell adhesion and spreading process, including the observation of cellular skeletal changes upon cell adhesion, the measurement of the expression level changes of cell adhesion, and the spreading specific genes and proteins upon exposure to CNTs.",
keywords = "Carbon nanotubes , HepG2 , Cell adhesion, Cell spreading , Cell viability, Transmission electron microscopy , Scanning electron microscopy reverse transcription polymerase chain reaction, Western immunoblotting ",
author = "Suijian Qi and Changqing Yi and Dawei Zhang and Mengsu Yang",
note = "Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).",
year = "2010",
doi = "10.1007/978-1-60761-579-8_15",
language = "English",
isbn = "978-1-60761-577-4",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "179--194",
editor = "Balasubramanian, {Kannan } and Burghard, {Marko }",
booktitle = "Carbon Nanotubes",
address = "United States",
}