Effect of α-Amylase, Papain, and Spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

18 Scopus Citations
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Author(s)

  • Davide Monaco
  • Meriem Fatnassi
  • Mohamed Hammadi
  • Touhami Khorchani
  • Giovanni Michele Lacalandra

Detail(s)

Original languageEnglish
Pages (from-to)5-9
Journal / PublicationResearch in Veterinary Science
Volume105
Online published9 Jan 2016
Publication statusPublished - Apr 2016
Externally publishedYes

Abstract

Ejaculates fromfive clinically healthy dromedary camels (Camelus dromedarius) were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid®) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris- Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid® containing Bromelain. At 120 min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination. Only the aliquots diluted with TCF containing Papain underwent complete liquefaction. Sperm motility decreased significantly during the observation times, except for the samples diluted with Spermfluid® (P = 0.005). Diluted samples showed different levels of agglutination, with the lowest being observed in the control and the highest in the Papain-treated samples. The viscosity of dromedary camel ejaculates could be effectively reduced by using the proteolytic enzyme Papain.

Research Area(s)

  • Agglutination, Camelus dromedarius, Liquefaction, Seminal plasma, Sperm motility, Viability

Citation Format(s)

Effect of α-Amylase, Papain, and Spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates. / Monaco, Davide; Fatnassi, Meriem; Padalino, Barbara; Hammadi, Mohamed; Khorchani, Touhami; Lacalandra, Giovanni Michele.

In: Research in Veterinary Science, Vol. 105, 04.2016, p. 5-9.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review