TY - JOUR
T1 - Effect of α-Amylase, Papain, and Spermfluid® treatments on viscosity and semen parameters of dromedary camel ejaculates
AU - Monaco, Davide
AU - Fatnassi, Meriem
AU - Padalino, Barbara
AU - Hammadi, Mohamed
AU - Khorchani, Touhami
AU - Lacalandra, Giovanni Michele
PY - 2016/4
Y1 - 2016/4
N2 - Ejaculates fromfive clinically healthy dromedary camels (Camelus dromedarius) were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid®) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris- Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid® containing Bromelain. At 120 min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination. Only the aliquots diluted with TCF containing Papain underwent complete liquefaction. Sperm motility decreased significantly during the observation times, except for the samples diluted with Spermfluid® (P = 0.005). Diluted samples showed different levels of agglutination, with the lowest being observed in the control and the highest in the Papain-treated samples. The viscosity of dromedary camel ejaculates could be effectively reduced by using the proteolytic enzyme Papain.
AB - Ejaculates fromfive clinically healthy dromedary camels (Camelus dromedarius) were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid®) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris- Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid® containing Bromelain. At 120 min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination. Only the aliquots diluted with TCF containing Papain underwent complete liquefaction. Sperm motility decreased significantly during the observation times, except for the samples diluted with Spermfluid® (P = 0.005). Diluted samples showed different levels of agglutination, with the lowest being observed in the control and the highest in the Papain-treated samples. The viscosity of dromedary camel ejaculates could be effectively reduced by using the proteolytic enzyme Papain.
KW - Agglutination
KW - Camelus dromedarius
KW - Liquefaction
KW - Seminal plasma
KW - Sperm motility
KW - Viability
UR - http://www.scopus.com/inward/record.url?scp=84976908772&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84976908772&origin=recordpage
U2 - 10.1016/j.rvsc.2016.01.003
DO - 10.1016/j.rvsc.2016.01.003
M3 - RGC 21 - Publication in refereed journal
C2 - 27033899
SN - 0034-5288
VL - 105
SP - 5
EP - 9
JO - Research in Veterinary Science
JF - Research in Veterinary Science
ER -