EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells

Anil Kumar Pulikkathodi; Indu Sarangadharan; Yi-Hong Chen; Gwo-Bin Lee; Yu-Lin Wang

doi:10.1149/08509.0015ecst

EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells

Anil Kumar Pulikkathodi
, Indu Sarangadharan
, Yi-Hong Chen
, Gwo-Bin Lee
, Yu-Lin Wang

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

2 Citations (Scopus)

Abstract

In the present work we have designed, fabricated and characterized an EDL FET biosensor for detection and enumeration of CTCs and to investigate of bioelectric signals of captured cells. We monitor transmembrane potential changes of the cell and use this methodology to study the functioning of ion channels. Our FET biosensor offers high sensitivity with single cell resolution, without any sample pre-treatments in physiological salt concentrations, since it is EDL gated. The investigation of cellular and sensor response in different test environments, showed that sensor response changes as the transmembrane potential of cell changes. This was confirmed by causing step depolarizations in cell and recording the corresponding sensor response. The effect of cadmium as a calcium channel blocker is investigated. This cell based EDL FET biosensor has bright prospects in the study of fundamental cell biology, cell based diagnostic platforms, and drug discovery and development.

Original language	English
Title of host publication	Solid-State Electronics and Photonics in Biology and Medicine 5
Editors	Y.-L. Wang, M. J. Deen, A. M. Hoff
Publisher	Electrochemical Society Inc.
Pages	15-23
ISBN (Print)	9781607685395, 9781607688372
DOIs	https://doi.org/10.1149/08509.0015ecst
Publication status	Published - May 2018
Externally published	Yes
Event	233rd Electrochemical Society (ECS) Meeting: Solid-State Electronics and Photonics in Biology and Medicine 5 - Seattle, United States Duration: 13 May 2018 → 17 May 2018 https://www.electrochem.org/233

Publication series

Name	ECS Transactions
Number	9
Volume	85
ISSN (Print)	1938-6737
ISSN (Electronic)	1938-5862

Conference

Conference	233rd Electrochemical Society (ECS) Meeting
Place	United States
City	Seattle
Period	13/05/18 → 17/05/18
Internet address	https://www.electrochem.org/233

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10.1149/08509.0015ecst

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Pulikkathodi, A. K., Sarangadharan, I., Chen, Y.-H., Lee, G.-B., & Wang, Y.-L. (2018). EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells. In Y.-L. Wang, M. J. Deen, & A. M. Hoff (Eds.), Solid-State Electronics and Photonics in Biology and Medicine 5 (pp. 15-23). (ECS Transactions; Vol. 85, No. 9). Electrochemical Society Inc.. https://doi.org/10.1149/08509.0015ecst

Pulikkathodi, Anil Kumar ; Sarangadharan, Indu ; Chen, Yi-Hong et al. / EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells. Solid-State Electronics and Photonics in Biology and Medicine 5. editor / Y.-L. Wang ; M. J. Deen ; A. M. Hoff. Electrochemical Society Inc., 2018. pp. 15-23 (ECS Transactions; 9).

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title = "EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells",

abstract = "In the present work we have designed, fabricated and characterized an EDL FET biosensor for detection and enumeration of CTCs and to investigate of bioelectric signals of captured cells. We monitor transmembrane potential changes of the cell and use this methodology to study the functioning of ion channels. Our FET biosensor offers high sensitivity with single cell resolution, without any sample pre-treatments in physiological salt concentrations, since it is EDL gated. The investigation of cellular and sensor response in different test environments, showed that sensor response changes as the transmembrane potential of cell changes. This was confirmed by causing step depolarizations in cell and recording the corresponding sensor response. The effect of cadmium as a calcium channel blocker is investigated. This cell based EDL FET biosensor has bright prospects in the study of fundamental cell biology, cell based diagnostic platforms, and drug discovery and development.",

author = "Pulikkathodi, \{Anil Kumar\} and Indu Sarangadharan and Yi-Hong Chen and Gwo-Bin Lee and Yu-Lin Wang",

year = "2018",

month = may,

doi = "10.1149/08509.0015ecst",

language = "English",

isbn = "9781607685395",

series = "ECS Transactions",

publisher = "Electrochemical Society Inc.",

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editor = "Y.-L. Wang and Deen, \{M. J.\} and Hoff, \{A. M.\}",

booktitle = "Solid-State Electronics and Photonics in Biology and Medicine 5",

note = "233rd Electrochemical Society (ECS) Meeting : Solid-State Electronics and Photonics in Biology and Medicine 5 ; Conference date: 13-05-2018 Through 17-05-2018",

url = "https://www.electrochem.org/233",

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Pulikkathodi, AK, Sarangadharan, I, Chen, Y-H, Lee, G-B & Wang, Y-L 2018, EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells. in Y-L Wang, MJ Deen & AM Hoff (eds), Solid-State Electronics and Photonics in Biology and Medicine 5. ECS Transactions, no. 9, vol. 85, Electrochemical Society Inc., pp. 15-23, 233rd Electrochemical Society (ECS) Meeting, Seattle, Washington, United States, 13/05/18. https://doi.org/10.1149/08509.0015ecst

EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells. / Pulikkathodi, Anil Kumar; Sarangadharan, Indu; Chen, Yi-Hong et al.
Solid-State Electronics and Photonics in Biology and Medicine 5. ed. / Y.-L. Wang; M. J. Deen; A. M. Hoff. Electrochemical Society Inc., 2018. p. 15-23 (ECS Transactions; Vol. 85, No. 9).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells

AU - Pulikkathodi, Anil Kumar

AU - Sarangadharan, Indu

AU - Chen, Yi-Hong

AU - Lee, Gwo-Bin

AU - Wang, Yu-Lin

PY - 2018/5

Y1 - 2018/5

N2 - In the present work we have designed, fabricated and characterized an EDL FET biosensor for detection and enumeration of CTCs and to investigate of bioelectric signals of captured cells. We monitor transmembrane potential changes of the cell and use this methodology to study the functioning of ion channels. Our FET biosensor offers high sensitivity with single cell resolution, without any sample pre-treatments in physiological salt concentrations, since it is EDL gated. The investigation of cellular and sensor response in different test environments, showed that sensor response changes as the transmembrane potential of cell changes. This was confirmed by causing step depolarizations in cell and recording the corresponding sensor response. The effect of cadmium as a calcium channel blocker is investigated. This cell based EDL FET biosensor has bright prospects in the study of fundamental cell biology, cell based diagnostic platforms, and drug discovery and development.

AB - In the present work we have designed, fabricated and characterized an EDL FET biosensor for detection and enumeration of CTCs and to investigate of bioelectric signals of captured cells. We monitor transmembrane potential changes of the cell and use this methodology to study the functioning of ion channels. Our FET biosensor offers high sensitivity with single cell resolution, without any sample pre-treatments in physiological salt concentrations, since it is EDL gated. The investigation of cellular and sensor response in different test environments, showed that sensor response changes as the transmembrane potential of cell changes. This was confirmed by causing step depolarizations in cell and recording the corresponding sensor response. The effect of cadmium as a calcium channel blocker is investigated. This cell based EDL FET biosensor has bright prospects in the study of fundamental cell biology, cell based diagnostic platforms, and drug discovery and development.

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85050239903&origin=recordpage

U2 - 10.1149/08509.0015ecst

DO - 10.1149/08509.0015ecst

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781607685395

SN - 9781607688372

T3 - ECS Transactions

SP - 15

EP - 23

BT - Solid-State Electronics and Photonics in Biology and Medicine 5

A2 - Wang, Y.-L.

A2 - Deen, M. J.

A2 - Hoff, A. M.

PB - Electrochemical Society Inc.

T2 - 233rd Electrochemical Society (ECS) Meeting

Y2 - 13 May 2018 through 17 May 2018

ER -

Pulikkathodi AK, Sarangadharan I, Chen YH, Lee GB, Wang YL. EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells. In Wang YL, Deen MJ, Hoff AM, editors, Solid-State Electronics and Photonics in Biology and Medicine 5. Electrochemical Society Inc. 2018. p. 15-23. (ECS Transactions; 9). doi: 10.1149/08509.0015ecst

EDL Gated FET Biosensor Array for the Investigation of Ion Channels and Bioelectric Signals of Circulating Tumor Cells

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