EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma

Xiaoming Lyu; Jianguo Wang; Xia Guo; Gongfa Wu; Yang Jiao; Oluwasijibomi Damola Faleti; Pengfei Liu; Tielian Liu; Yufei Long; Tuotuo Chong; Xu Yang; Jing Huang; Mingliang He; Chi Man Tsang; Sai Wah Tsao; Qian Wang; Qiang Jiang; Xin Li

doi:10.1371/journal.ppat.1007484

EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma

Xiaoming Lyu^*
, Jianguo Wang
, Xia Guo
, Gongfa Wu
, Yang Jiao
, Oluwasijibomi Damola Faleti
, Pengfei Liu
, Tielian Liu
, Yufei Long
, Tuotuo Chong
, Xu Yang
, Jing Huang
, Mingliang He
, Chi Man Tsang
, Sai Wah Tsao
, Qian Wang
, Qiang Jiang^*
, Xin Li^*

^*Corresponding author for this work

Department of Biomedical Sciences

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

98 Citations (Scopus)

111 Downloads (CityUHK Scholars)

Abstract

Abnormal metabolism and uncontrolled angiogenesis are two important characteristics of malignant tumors. The occurrence of both events involves many key molecular changes including miRNA. However, EBV encoded miRNAs are rarely mentioned as capable of regulating tumor metabolism and tumor angiogenesis. Here, we reported that one of the key miRNAs encoded by EBV, EBV-miR-Bart1-5P, can significantly promote nasopharyngeal carcinoma (NPC) cell glycolysis and induces angiogenesis in vitro and in vivo. Mechanistically, EBV-miR-Bart1-5P directly targets the α1 catalytic subunit of AMP-activated protein kinase (AMPKα1) and consequently regulates the AMPK/mTOR/HIF1 pathway which impelled NPC cell anomalous aerobic glycolysis and angiogenesis, ultimately leads to uncontrolled growth of NPC. Our findings provide new insights into metabolism and angiogenesis of NPC and new opportunities for the development of targeted NPC therapy in the future.

Original language	English
Article number	e1007484
Journal	PLoS Pathogens
Volume	14
Issue number	12
Online published	17 Dec 2018
DOIs	https://doi.org/10.1371/journal.ppat.1007484
Publication status	Published - Dec 2018

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Lyu, X., Wang, J., Guo, X., Wu, G., Jiao, Y., Faleti, O. D., Liu, P., Liu, T., Long, Y., Chong, T., Yang, X., Huang, J., He, M., Tsang, C. M., Tsao, S. W., Wang, Q., Jiang, Q., & Li, X. (2018). EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma. PLoS Pathogens, 14(12), Article e1007484. https://doi.org/10.1371/journal.ppat.1007484

@article{98afa3f0ddd94478a30a820161c8faa2,

title = "EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma",

abstract = "Abnormal metabolism and uncontrolled angiogenesis are two important characteristics of malignant tumors. The occurrence of both events involves many key molecular changes including miRNA. However, EBV encoded miRNAs are rarely mentioned as capable of regulating tumor metabolism and tumor angiogenesis. Here, we reported that one of the key miRNAs encoded by EBV, EBV-miR-Bart1-5P, can significantly promote nasopharyngeal carcinoma (NPC) cell glycolysis and induces angiogenesis in vitro and in vivo. Mechanistically, EBV-miR-Bart1-5P directly targets the α1 catalytic subunit of AMP-activated protein kinase (AMPKα1) and consequently regulates the AMPK/mTOR/HIF1 pathway which impelled NPC cell anomalous aerobic glycolysis and angiogenesis, ultimately leads to uncontrolled growth of NPC. Our findings provide new insights into metabolism and angiogenesis of NPC and new opportunities for the development of targeted NPC therapy in the future.",

author = "Xiaoming Lyu and Jianguo Wang and Xia Guo and Gongfa Wu and Yang Jiao and Faleti, \{Oluwasijibomi Damola\} and Pengfei Liu and Tielian Liu and Yufei Long and Tuotuo Chong and Xu Yang and Jing Huang and Mingliang He and Tsang, \{Chi Man\} and Tsao, \{Sai Wah\} and Qian Wang and Qiang Jiang and Xin Li",

year = "2018",

month = dec,

doi = "10.1371/journal.ppat.1007484",

language = "English",

volume = "14",

journal = "PLoS Pathogens",

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publisher = "Public Library of Science (PLoS)",

number = "12",

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Lyu, X, Wang, J, Guo, X, Wu, G, Jiao, Y , Faleti, OD, Liu, P, Liu, T, Long, Y, Chong, T, Yang, X, Huang, J, He, M, Tsang, CM, Tsao, SW, Wang, Q, Jiang, Q & Li, X 2018, 'EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma', PLoS Pathogens, vol. 14, no. 12, e1007484. https://doi.org/10.1371/journal.ppat.1007484

TY - JOUR

T1 - EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma

AU - Lyu, Xiaoming

AU - Wang, Jianguo

AU - Guo, Xia

AU - Wu, Gongfa

AU - Jiao, Yang

AU - Faleti, Oluwasijibomi Damola

AU - Liu, Pengfei

AU - Liu, Tielian

AU - Long, Yufei

AU - Chong, Tuotuo

AU - Yang, Xu

AU - Huang, Jing

AU - He, Mingliang

AU - Tsang, Chi Man

AU - Tsao, Sai Wah

AU - Wang, Qian

AU - Jiang, Qiang

AU - Li, Xin

PY - 2018/12

Y1 - 2018/12

N2 - Abnormal metabolism and uncontrolled angiogenesis are two important characteristics of malignant tumors. The occurrence of both events involves many key molecular changes including miRNA. However, EBV encoded miRNAs are rarely mentioned as capable of regulating tumor metabolism and tumor angiogenesis. Here, we reported that one of the key miRNAs encoded by EBV, EBV-miR-Bart1-5P, can significantly promote nasopharyngeal carcinoma (NPC) cell glycolysis and induces angiogenesis in vitro and in vivo. Mechanistically, EBV-miR-Bart1-5P directly targets the α1 catalytic subunit of AMP-activated protein kinase (AMPKα1) and consequently regulates the AMPK/mTOR/HIF1 pathway which impelled NPC cell anomalous aerobic glycolysis and angiogenesis, ultimately leads to uncontrolled growth of NPC. Our findings provide new insights into metabolism and angiogenesis of NPC and new opportunities for the development of targeted NPC therapy in the future.

AB - Abnormal metabolism and uncontrolled angiogenesis are two important characteristics of malignant tumors. The occurrence of both events involves many key molecular changes including miRNA. However, EBV encoded miRNAs are rarely mentioned as capable of regulating tumor metabolism and tumor angiogenesis. Here, we reported that one of the key miRNAs encoded by EBV, EBV-miR-Bart1-5P, can significantly promote nasopharyngeal carcinoma (NPC) cell glycolysis and induces angiogenesis in vitro and in vivo. Mechanistically, EBV-miR-Bart1-5P directly targets the α1 catalytic subunit of AMP-activated protein kinase (AMPKα1) and consequently regulates the AMPK/mTOR/HIF1 pathway which impelled NPC cell anomalous aerobic glycolysis and angiogenesis, ultimately leads to uncontrolled growth of NPC. Our findings provide new insights into metabolism and angiogenesis of NPC and new opportunities for the development of targeted NPC therapy in the future.

UR - https://www.scopus.com/pages/publications/85059290598

UR - https://www.scopus.com/pages/publications/85059290598#tab=impact

U2 - 10.1371/journal.ppat.1007484

DO - 10.1371/journal.ppat.1007484

M3 - RGC 21 - Publication in refereed journal

C2 - 30557400

SN - 1553-7366

VL - 14

JO - PLoS Pathogens

JF - PLoS Pathogens

IS - 12

M1 - e1007484

ER -

EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma

Abstract

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Correction: EBV-miR-BART1-5P activates AMPK/mTOR/HIF1 pathway via a PTEN independent manner to promote glycolysis and angiogenesis in nasopharyngeal carcinoma

Cite this