Dynamics of natural killer cells cytotoxicity in microwell arrays with connecting channels

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publicationBiomaterials 2016
Pages50
Publication statusPublished - Mar 2016

Publication series

NameJournal of Biotechnology & Biomaterials
Number1 (Supp.)
Volume6
ISSN (Electronic)2155-952X

Conference

TitleAnnual Conference and Expo on Biomaterials 2016
PlaceUnited Kingdom
CityLondon
Period14 - 16 March 2016

Abstract

Natural killer (NK) cells serve an important role in immune system by recognizing and killing the potentially malignant cells without antigen sensitization, and could be promising in cancer therapy. We have designed and fabricated microwell arrays with microchannel connections in polydimethylsiloxane (PDMS) substrates to study the interaction dynamics of NK-92MI cells with MCF7 breast cancer cells using time-lapse imaging by fluorescence microscopy for 15 h. Although cell seeding density was the same, NK cell cytotoxicity was found to be higher in larger microwells, which is manifested as increased target death ratio from 13.7 ± 3.1 to 46.3 ± 3.3% and shorter triggering time of first target lysis from 502 ± 49 to 391 ± 63 min in 150 μm × 150 μm microwells comparing to 50 μm × 50 μm wells in 15 h. Mirochannel connection between adjacent microwells of the same size increased the overall target death ratio by >10%, while connection between microwells of different sizes led to significantly increased target death ratio and delayed first target lysis in smaller microwells. Our findings reveal unique cell interaction dynamics, such as initiation and stimulation, of NK cell cytotoxicity in a confined microenvironment, which is different from population-based study, and the results could lead to a better understanding of the dynamics of NK cell cytotoxicity.

Research Area(s)

  • natural killer cells, microwells, microchannels, cytotoxicity, cell interactions

Bibliographic Note

“Full text of this publication does not contain sufficient affiliation information. With consent from the author(s) concerned, the Research Unit(s) information for this record is based on the existing academic department affiliation of the author(s).

Citation Format(s)

Dynamics of natural killer cells cytotoxicity in microwell arrays with connecting channels. / Yuanhao, Xu; Zhou, S F; Gopalakrishnan, S et al.
Biomaterials 2016. 2016. p. 50 (Journal of Biotechnology & Biomaterials; Vol. 6, No. 1 (Supp.)).

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review