Dynamics of mitochondria and mitochondrial Ca2+ near the plasma membrane of PC12 cells: A study by multimode microscopy

De-Ming Yang; Chung-Chih Lin; Hsia Yu Lin; Chien-Chang Huang; Din Ping Tsai; Chin-Wen Chi; Lung-Sen Kao

doi:10.1196/annals.1338.018

Dynamics of mitochondria and mitochondrial Ca²⁺ near the plasma membrane of PC12 cells: A study by multimode microscopy

De-Ming Yang
, Chung-Chih Lin
, Hsia Yu Lin
, Chien-Chang Huang
, Din Ping Tsai
, Chin-Wen Chi
, Lung-Sen Kao

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

2 Citations (Scopus)

Abstract

The goal of this study is to examine whether there is a difference in the regulation of Ca²⁺ between mitochondria near the cell surface and mitochondria in the cytosol. Total internal reflection fluorescence and epifluorescence microscopy were used to monitor changes in the mitochondrial Ca²⁺ ([Ca²⁺]_mt,) between the mitochondria near the plasma membrane and those in the cytosol. The results show that [Ca ²⁺]_mt near the plasma membrane increased earlier and decayed slower after high K⁺ stimulation than average mitochondria in the cytosol. In addition, the changes in [Ca²⁺]_mt in the mitochondria near the cell surface after a second stimulation were larger than those induced by the first stimulation. The results provide direct evidence to support the hypothesis that mitochondria in different subcellular localization show differential responses to the influx of extracellular Ca²⁺. © 2005 New York Academy of Sciences.

Original language	English
Pages (from-to)	163-167
Journal	Annals of the New York Academy of Sciences
Volume	1042
DOIs	https://doi.org/10.1196/annals.1338.018
Publication status	Published - 2005
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Research Keywords

Mitochondria
Mitochondrial Ca2+
PC12 cells
Total internal reflection fluorescence microscopy

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Cite this

@article{156b984d53114a6b963533cd884e2133,

title = "Dynamics of mitochondria and mitochondrial Ca2+ near the plasma membrane of PC12 cells: A study by multimode microscopy",

abstract = "The goal of this study is to examine whether there is a difference in the regulation of Ca2+ between mitochondria near the cell surface and mitochondria in the cytosol. Total internal reflection fluorescence and epifluorescence microscopy were used to monitor changes in the mitochondrial Ca2+ ([Ca2+]mt,) between the mitochondria near the plasma membrane and those in the cytosol. The results show that [Ca 2+]mt near the plasma membrane increased earlier and decayed slower after high K+ stimulation than average mitochondria in the cytosol. In addition, the changes in [Ca2+]mt in the mitochondria near the cell surface after a second stimulation were larger than those induced by the first stimulation. The results provide direct evidence to support the hypothesis that mitochondria in different subcellular localization show differential responses to the influx of extracellular Ca2+. {\textcopyright} 2005 New York Academy of Sciences.",

keywords = "Mitochondria, Mitochondrial Ca2+, PC12 cells, Total internal reflection fluorescence microscopy",

author = "De-Ming Yang and Chung-Chih Lin and Lin, \{Hsia Yu\} and Chien-Chang Huang and Tsai, \{Din Ping\} and Chin-Wen Chi and Lung-Sen Kao",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected]. ",

year = "2005",

doi = "10.1196/annals.1338.018",

language = "English",

volume = "1042",

pages = "163--167",

journal = "Annals of the New York Academy of Sciences",

issn = "0077-8923",

publisher = "John Wiley \& Sons",

}

TY - JOUR

T1 - Dynamics of mitochondria and mitochondrial Ca2+ near the plasma membrane of PC12 cells

T2 - A study by multimode microscopy

AU - Yang, De-Ming

AU - Lin, Chung-Chih

AU - Lin, Hsia Yu

AU - Huang, Chien-Chang

AU - Tsai, Din Ping

AU - Chi, Chin-Wen

AU - Kao, Lung-Sen

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2005

Y1 - 2005

N2 - The goal of this study is to examine whether there is a difference in the regulation of Ca2+ between mitochondria near the cell surface and mitochondria in the cytosol. Total internal reflection fluorescence and epifluorescence microscopy were used to monitor changes in the mitochondrial Ca2+ ([Ca2+]mt,) between the mitochondria near the plasma membrane and those in the cytosol. The results show that [Ca 2+]mt near the plasma membrane increased earlier and decayed slower after high K+ stimulation than average mitochondria in the cytosol. In addition, the changes in [Ca2+]mt in the mitochondria near the cell surface after a second stimulation were larger than those induced by the first stimulation. The results provide direct evidence to support the hypothesis that mitochondria in different subcellular localization show differential responses to the influx of extracellular Ca2+. © 2005 New York Academy of Sciences.

AB - The goal of this study is to examine whether there is a difference in the regulation of Ca2+ between mitochondria near the cell surface and mitochondria in the cytosol. Total internal reflection fluorescence and epifluorescence microscopy were used to monitor changes in the mitochondrial Ca2+ ([Ca2+]mt,) between the mitochondria near the plasma membrane and those in the cytosol. The results show that [Ca 2+]mt near the plasma membrane increased earlier and decayed slower after high K+ stimulation than average mitochondria in the cytosol. In addition, the changes in [Ca2+]mt in the mitochondria near the cell surface after a second stimulation were larger than those induced by the first stimulation. The results provide direct evidence to support the hypothesis that mitochondria in different subcellular localization show differential responses to the influx of extracellular Ca2+. © 2005 New York Academy of Sciences.

KW - Mitochondria

KW - Mitochondrial Ca2+

KW - PC12 cells

KW - Total internal reflection fluorescence microscopy

UR - https://www.scopus.com/pages/publications/22044445989

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-22044445989&origin=recordpage

U2 - 10.1196/annals.1338.018

DO - 10.1196/annals.1338.018

M3 - RGC 21 - Publication in refereed journal

C2 - 15965059

SN - 0077-8923

VL - 1042

SP - 163

EP - 167

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

ER -