Dynamically Tunable, Macroscopic Molecular Networks Enabled by Cellular Synthesis of 4-Arm Star-like Proteins

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Zhongguang Yang
  • Yang Yang
  • Mo Wang
  • Hong Kiu Francis Fok
  • Bojing Jiang
  • Wendi Xiao
  • Songzi Kou
  • Yusong Guo
  • Yan Yan
  • Wen-Bin Zhang
  • Fei Sun

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)233-249
Journal / PublicationMatter
Volume2
Issue number1
Online published9 Oct 2019
Publication statusPublished - 8 Jan 2020

Abstract

Design of proteins with nonlinear topologies has emerged as a nascent branch of protein engineering, but significant applications remain to be seen. Here, we demonstrate the cellular synthesis of (SpyCatcher)4GFP, a 4-arm star-like protein enabled by spontaneous split GFP reconstitution, which further led to the creation of various protein networks exhibiting tunable mechanics and suitability for cell encapsulation. A derivative 4-arm star-like protein, (CarHC)4GFP, resulting from the conjugation of (SpyCatcher)4GFP with the SpyTag-fusion CarHC photoreceptors, can undergo rapid sol-gel and gel-sol transitions in response to AdoB12 and light, respectively. The chemo- and photo-induced phase transitions enabled encapsulation and controlled release of protein molecules such as the biofilm-degrading glycosyl hydrolase PslG, a potential agent for combatting multidrug-resistant bacterial species in chronic infections. The creation of those uncommon protein architectures promises great opportunities for materials biology and “smart” therapeutic delivery. 

Research Area(s)

  • 3D cell culture, biofilm inhibition, cell recovery/release, MAP4: demonstrate, molecular engineering, phase transition, photoresponsive hydrogel, protein topology engineering, split GFP reconstitution, SpyTag/SpyCatcher, synthetic biology

Citation Format(s)

Dynamically Tunable, Macroscopic Molecular Networks Enabled by Cellular Synthesis of 4-Arm Star-like Proteins. / Yang, Zhongguang; Yang, Yang; Wang, Mo; Wang, Tingting; Fok, Hong Kiu Francis; Jiang, Bojing; Xiao, Wendi; Kou, Songzi; Guo, Yusong; Yan, Yan; Deng, Xin; Zhang, Wen-Bin; Sun, Fei.

In: Matter, Vol. 2, No. 1, 08.01.2020, p. 233-249.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review