Dynamic Surface Proteomes of Allergenic Fungal Conidia

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Matthew G Blango
  • Annica Pschibul
  • Flora Rivieccio
  • Thomas Krüger
  • Muhammad Rafiq
  • Lei-Jie Jia
  • Tingting Zheng
  • Marie Goldmann
  • Vera Voltersen
  • Gianni Panagiotou
  • Olaf Kniemeyer
  • Axel A. Brakhage

Detail(s)

Original languageEnglish
Pages (from-to)2092-2104
Journal / PublicationJournal of Proteome Research
Volume19
Issue number5
Online published1 Apr 2020
Publication statusPublished - 1 May 2020

Abstract

Fungal spores and hyphal fragments play an important role as allergens in respiratory diseases. In this study, we performed trypsin shaving and secretome analyses to identify the surface-exposed proteins and secreted/shed proteins of Aspergillus fumigatus conidia, respectively. We investigated the surface proteome under different conditions, including temperature variation and germination. We found that the surface proteome of resting A. fumigatus conidia is not static but instead unexpectedly dynamic, as evidenced by drastically different surface proteomes under different growth conditions. Knockouts of two abundant A. fumigatus surface proteins, ScwA and CweA, were found to function only in fine-tuning the cell wall stress response, implying that the conidial surface is very robust against perturbations. We then compared the surface proteome of A. fumigatus to other allergy-inducing molds, including Alternaria alternata, Penicillium rubens, and Cladosporium herbarum, and performed comparative proteomics on resting and swollen conidia, as well as secreted proteins from germinating conidia. We detected 125 protein ortholog groups, including 80 with putative catalytic activity, in the extracellular region of all four molds, and 42 nonorthologous proteins produced solely by A. fumigatus. Ultimately, this study highlights the dynamic nature of the A. fumigatus conidial surface and provides targets for future diagnostics and immunotherapy.

Research Area(s)

  • Aspergillus fumigatus, Alternaria alternata, Penicillium rubens, Cladosporium herbarum, proteome, spore, germling, hyphae, LC−MS/MS, secreted proteins

Citation Format(s)

Dynamic Surface Proteomes of Allergenic Fungal Conidia. / Blango, Matthew G; Pschibul, Annica; Rivieccio, Flora; Krüger, Thomas; Rafiq, Muhammad; Jia, Lei-Jie; Zheng, Tingting; Goldmann, Marie; Voltersen, Vera; Li, Jun; Panagiotou, Gianni; Kniemeyer, Olaf; Brakhage, Axel A.

In: Journal of Proteome Research, Vol. 19, No. 5, 01.05.2020, p. 2092-2104.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal