Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics

Wenfeng Liang; Bin Liu; Lianqing Liu; Zaili Dong; Gwo-Bin Lee; Wen J. Li; Weijing Zhang

doi:10.1109/NEMS.2013.6559946

Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics

Wenfeng Liang
, Bin Liu
, Lianqing Liu
, Zaili Dong
, Gwo-Bin Lee
, Wen J. Li^*
, Weijing Zhang

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

2 Citations (Scopus)

Abstract

This study reports an approach to dynamically separate cultured human B-lymphoma (Raji) cells from normal human red blood cells (RBCs), mixed in an isotonic solution, by utilizing a hydrogenated amorphous silicon-based optically-induced electrokinetics chip. A theoretical calculation of the polarization model for the two different types of cells is first presented. The differential velocity between the cell types, generated as a result of the optically-induced dielectrophoresis (ODEP) force is obtained using a finite element method. The theoretical results indicate that the two types of cells could be separated by using the ODEP force. Experimental results verified that the two types of cells can be separated by using two optically projected lines of different widths, with one projected line moving and the other line stationary, as the virtual electrodes for generating the ODEP force. Separation is achieved with these virtual electrodes using a bias potential of 20 V _pp at a driving frequency of 50 kHz. To the best of our knowledge, this work is the first experimental validation of rapid separation of Raji cells from RBCs using optically-induced electrokinetics. © 2013 IEEE.

Original language	English
Title of host publication	8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013
Pages	1253-1256
DOIs	https://doi.org/10.1109/NEMS.2013.6559946
Publication status	Published - 2013
Event	8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013) - Suzhou, China Duration: 7 Apr 2013 → 10 Apr 2013 http://cnm.cust.edu.cn/en/node/26

Conference

Conference	8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013)
Abbreviated title	NEMS 2013
Place	China
City	Suzhou
Period	7/04/13 → 10/04/13
Internet address	http://cnm.cust.edu.cn/en/node/26

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

B-lymphoma cells
dielectrophoresis
optically-induced dielectrophoresis
red blood cells

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10.1109/NEMS.2013.6559946

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@inproceedings{f9c7f02d8da046bdb396aa25c5029b67,

title = "Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics",

abstract = "This study reports an approach to dynamically separate cultured human B-lymphoma (Raji) cells from normal human red blood cells (RBCs), mixed in an isotonic solution, by utilizing a hydrogenated amorphous silicon-based optically-induced electrokinetics chip. A theoretical calculation of the polarization model for the two different types of cells is first presented. The differential velocity between the cell types, generated as a result of the optically-induced dielectrophoresis (ODEP) force is obtained using a finite element method. The theoretical results indicate that the two types of cells could be separated by using the ODEP force. Experimental results verified that the two types of cells can be separated by using two optically projected lines of different widths, with one projected line moving and the other line stationary, as the virtual electrodes for generating the ODEP force. Separation is achieved with these virtual electrodes using a bias potential of 20 V pp at a driving frequency of 50 kHz. To the best of our knowledge, this work is the first experimental validation of rapid separation of Raji cells from RBCs using optically-induced electrokinetics. {\textcopyright} 2013 IEEE.",

keywords = "B-lymphoma cells, dielectrophoresis, optically-induced dielectrophoresis, red blood cells",

author = "Wenfeng Liang and Bin Liu and Lianqing Liu and Zaili Dong and Gwo-Bin Lee and Li, \{Wen J.\} and Weijing Zhang",

year = "2013",

doi = "10.1109/NEMS.2013.6559946",

language = "English",

isbn = "9781467363525",

pages = "1253--1256",

booktitle = "8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013",

note = "8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013), NEMS 2013 ; Conference date: 07-04-2013 Through 10-04-2013",

url = "http://cnm.cust.edu.cn/en/node/26",

}

Liang, W, Liu, B, Liu, L, Dong, Z, Lee, G-B, Li, WJ & Zhang, W 2013, Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics. in 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013., 6559946, pp. 1253-1256, 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013), Suzhou, China, 7/04/13. https://doi.org/10.1109/NEMS.2013.6559946

Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics. / Liang, Wenfeng; Liu, Bin; Liu, Lianqing et al.
8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013. 2013. p. 1253-1256 6559946.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics

AU - Liang, Wenfeng

AU - Liu, Bin

AU - Liu, Lianqing

AU - Dong, Zaili

AU - Lee, Gwo-Bin

AU - Li, Wen J.

AU - Zhang, Weijing

PY - 2013

Y1 - 2013

N2 - This study reports an approach to dynamically separate cultured human B-lymphoma (Raji) cells from normal human red blood cells (RBCs), mixed in an isotonic solution, by utilizing a hydrogenated amorphous silicon-based optically-induced electrokinetics chip. A theoretical calculation of the polarization model for the two different types of cells is first presented. The differential velocity between the cell types, generated as a result of the optically-induced dielectrophoresis (ODEP) force is obtained using a finite element method. The theoretical results indicate that the two types of cells could be separated by using the ODEP force. Experimental results verified that the two types of cells can be separated by using two optically projected lines of different widths, with one projected line moving and the other line stationary, as the virtual electrodes for generating the ODEP force. Separation is achieved with these virtual electrodes using a bias potential of 20 V pp at a driving frequency of 50 kHz. To the best of our knowledge, this work is the first experimental validation of rapid separation of Raji cells from RBCs using optically-induced electrokinetics. © 2013 IEEE.

AB - This study reports an approach to dynamically separate cultured human B-lymphoma (Raji) cells from normal human red blood cells (RBCs), mixed in an isotonic solution, by utilizing a hydrogenated amorphous silicon-based optically-induced electrokinetics chip. A theoretical calculation of the polarization model for the two different types of cells is first presented. The differential velocity between the cell types, generated as a result of the optically-induced dielectrophoresis (ODEP) force is obtained using a finite element method. The theoretical results indicate that the two types of cells could be separated by using the ODEP force. Experimental results verified that the two types of cells can be separated by using two optically projected lines of different widths, with one projected line moving and the other line stationary, as the virtual electrodes for generating the ODEP force. Separation is achieved with these virtual electrodes using a bias potential of 20 V pp at a driving frequency of 50 kHz. To the best of our knowledge, this work is the first experimental validation of rapid separation of Raji cells from RBCs using optically-induced electrokinetics. © 2013 IEEE.

KW - B-lymphoma cells

KW - dielectrophoresis

KW - optically-induced dielectrophoresis

KW - red blood cells

UR - https://www.scopus.com/pages/publications/84883109765

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84883109765&origin=recordpage

U2 - 10.1109/NEMS.2013.6559946

DO - 10.1109/NEMS.2013.6559946

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781467363525

SP - 1253

EP - 1256

BT - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems, IEEE NEMS 2013

T2 - 8th Annual IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS 2013)

Y2 - 7 April 2013 through 10 April 2013

ER -

Dynamic separation of b-lymphoma cells from red blood cells using optically-induced electrokinetics

Abstract

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UN SDGs

Research Keywords

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