TY - JOUR
T1 - Dynamic monitoring of transmembrane potential changes
T2 - a study of ion channels using an electrical double layer-gated FET biosensor
AU - Pulikkathodi, Anil Kumar
AU - Sarangadharan, Indu
AU - Chen, Yi-Hong
AU - Lee, Geng-Yen
AU - Chyi, Jen-Inn
AU - Lee, Gwo-Bin
AU - Wang, Yu-Lin
PY - 2018/4/7
Y1 - 2018/4/7
N2 - In this research, we have designed, fabricated and characterized an electrical double layer (EDL)-gated AlGaN/GaN high electron mobility transistor (HEMT) biosensor array to study the transmembrane potential changes of cells. The sensor array platform is designed to detect and count circulating tumor cells (CTCs) of colorectal cancer (CRC) and investigate cellular bioelectric signals. Using the EDL FET biosensor platform, cellular responses can be studied in physiological salt concentrations, thereby eliminating complex automation. Upon investigation, we discovered that our sensor response follows the transmembrane potential changes of captured cells. Our whole cell sensor platform can be used to monitor the dynamic changes in the membrane potential of cells. The effects of continuously changing electrolyte ion concentrations and ion channel blocking using cadmium are investigated. This methodology has the potential to be used as an electrophysiological probe for studying ion channel gating and the interaction of biomolecules in cells. The sensor can also be a point-of-care diagnostic tool for rapid screening of diseases.
AB - In this research, we have designed, fabricated and characterized an electrical double layer (EDL)-gated AlGaN/GaN high electron mobility transistor (HEMT) biosensor array to study the transmembrane potential changes of cells. The sensor array platform is designed to detect and count circulating tumor cells (CTCs) of colorectal cancer (CRC) and investigate cellular bioelectric signals. Using the EDL FET biosensor platform, cellular responses can be studied in physiological salt concentrations, thereby eliminating complex automation. Upon investigation, we discovered that our sensor response follows the transmembrane potential changes of captured cells. Our whole cell sensor platform can be used to monitor the dynamic changes in the membrane potential of cells. The effects of continuously changing electrolyte ion concentrations and ion channel blocking using cadmium are investigated. This methodology has the potential to be used as an electrophysiological probe for studying ion channel gating and the interaction of biomolecules in cells. The sensor can also be a point-of-care diagnostic tool for rapid screening of diseases.
UR - http://www.scopus.com/inward/record.url?scp=85044672089&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85044672089&origin=recordpage
U2 - 10.1039/c7lc01305a
DO - 10.1039/c7lc01305a
M3 - RGC 21 - Publication in refereed journal
C2 - 29488525
SN - 1473-0197
VL - 18
SP - 1047
EP - 1056
JO - Lab on a Chip
JF - Lab on a Chip
IS - 7
ER -
