Dynamic Localisation of Mature MicroRNAs in Human Nucleoli is Influenced by Exogenous Genetic Materials

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

35 Scopus Citations
View graph of relations

Author(s)

  • Zhou Fang Li
  • Yi Min Liang
  • Pui Ngan Lau
  • Wei Shen
  • Dai Kui Wang
  • Wing Tai Cheung
  • Lit Man Poon

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article numbere70869
Journal / PublicationPLoS ONE
Volume8
Issue number8
Publication statusPublished - 6 Aug 2013

Link(s)

Abstract

Although microRNAs are commonly known to function as a component of RNA-induced silencing complexes in the cytoplasm, they have been detected in other organelles, notably the nucleus and the nucleolus, of mammalian cells. We have conducted a systematic search for miRNAs in HeLa cell nucleoli, and identified 11 abundant miRNAs with a high level of nucleolar accumulation. Through in situ hybridisation, we have localised these miRNAs, including miR-191 and miR-484, in the nucleolus of a diversity of human and rodent cell lines. The nucleolar association of these miRNAs is resistant to various cellular stresses, but highly sensitive to the presence of exogenous nucleic acids. Introduction of both single- and double-stranded DNA as well as double stranded RNA rapidly induce the redistribution of nucleolar miRNAs to the cytoplasm. A similar change in subcellular distribution is also observed in cells infected with the influenza A virus. The partition of miRNAs between the nucleolus and the cytoplasm is affected by Leptomycin B, suggesting a role of Exportin-1 in the intracellular shuttling of miRNAs. This study reveals a previously unknown aspect of miRNA biology, and suggests a possible link between these small noncoding RNAs and the cellular management of foreign genetic materials. © 2013 Li et al.

Research Area(s)

Citation Format(s)

Dynamic Localisation of Mature MicroRNAs in Human Nucleoli is Influenced by Exogenous Genetic Materials. / Li, Zhou Fang; Liang, Yi Min; Lau, Pui Ngan; Shen, Wei; Wang, Dai Kui; Cheung, Wing Tai; Xue, Chun Jason; Poon, Lit Man; Lam, Yun Wah.

In: PLoS ONE, Vol. 8, No. 8, e70869, 06.08.2013.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

Download Statistics

No data available