Dynamic force generation by neural stem cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

16 Scopus Citations
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Author(s)

  • P. Shi
  • K. Shen
  • S. Ghassemi
  • J. Hone
  • L. C. Kam

Detail(s)

Original languageEnglish
Pages (from-to)464-474
Journal / PublicationCellular and Molecular Bioengineering
Volume2
Issue number4
Publication statusPublished - Dec 2009
Externally publishedYes

Abstract

Mechanical cues may have important roles in tissue morphogenesis; progression through complex functions like differentiation may be associated with changes in cellular force generation and mechanosensing. To explore this concept, we use elastomer pillar arrays to map forces generated by neural stem cells in vitro, and identify two distinct dynamics of force generation. First, cell generated forces decrease as cells transition from a proliferative mode to differentiation, a process covering several days. This change in force generation correlates with a loss of sensitivity to substrate rigidity over a series of polydimethylsiloxane substrates. Second, neural stem cells exhibit a faster pattern of localized contractions at the cell body and outlying processes; each lasts on the order of minutes, and is not synchronized across the cell. This faster process is reminiscent of migratory behavior observed in vivo, and may be involved in controlling the motion of internal structures such as the cell nucleus. These results together provide new clues into the role of forces during development, and may lead to design principles for materials targeted for use in the central nervous system. © 2009 Biomedical Engineering Society.

Research Area(s)

  • Cellular traction force, Differentiation, Microenvironment, Proliferation, Stem cell

Citation Format(s)

Dynamic force generation by neural stem cells. / Shi, P.; Shen, K.; Ghassemi, S.; Hone, J.; Kam, L. C.

In: Cellular and Molecular Bioengineering, Vol. 2, No. 4, 12.2009, p. 464-474.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review