TY - GEN
T1 - Dynamic control of cell migration using optical tweezers and microfluidic channel
AU - Gou, Xue
AU - Wang, Xiaolin
AU - Yang, Hao
AU - Yan, Xiao
AU - Wang, Yong
AU - Fahmy, Tarek M.
AU - Sun, Dong
PY - 2012
Y1 - 2012
N2 - This paper presents the development of a single-cell motility assay method that allows the quantification of cell chemotaxis under complex hydrodynamic condition. The specially designed microparticles, which contain cytokines and chemokines, are trapped by the optical tweezers in motion while releasing the chemical to stimulate the cell polarization and migration. The morphology, localization, and migration speed of the cell are examined quantitatively under different shear stresses. Experiments of migrating leukemia cells led by two microparticles releasing SDF-1a are performed to demonstrate the effectiveness of the proposed approach. This method will provide a new opportunity to probe the mechanism of cell migration at molecular and nano-scale precision level, and help clinicians to develop new targeted therapies in nanomedicine. © 2012 IEEE.
AB - This paper presents the development of a single-cell motility assay method that allows the quantification of cell chemotaxis under complex hydrodynamic condition. The specially designed microparticles, which contain cytokines and chemokines, are trapped by the optical tweezers in motion while releasing the chemical to stimulate the cell polarization and migration. The morphology, localization, and migration speed of the cell are examined quantitatively under different shear stresses. Experiments of migrating leukemia cells led by two microparticles releasing SDF-1a are performed to demonstrate the effectiveness of the proposed approach. This method will provide a new opportunity to probe the mechanism of cell migration at molecular and nano-scale precision level, and help clinicians to develop new targeted therapies in nanomedicine. © 2012 IEEE.
UR - http://www.scopus.com/inward/record.url?scp=84869183835&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84869183835&origin=recordpage
U2 - 10.1109/NANO.2012.6321983
DO - 10.1109/NANO.2012.6321983
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781467321983
BT - Proceedings of the IEEE Conference on Nanotechnology
T2 - 12th IEEE International Conference on Nanotechnology (IEEE NANO 2012)
Y2 - 20 August 2012 through 23 August 2012
ER -
