Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Jiangbo Chen; Yachao Zhang; Songnan Bai; Jingyi Zhu; Pakpong Chirarattananon; Kai Ni; Qian Zhou; Lidai Wang

doi:10.1016/j.pacs.2021.100292

Author(s)

Kai Ni
Qian Zhou
Lidai Wang

Related Research Unit(s)

Detail(s)

Original language	English
Article number	100292
Journal / Publication	Photoacoustics
Volume	23
Online published	9 Aug 2021
Publication status	Published - Sept 2021

Link(s)

DOI	DOI https://doi.org/10.1016/j.pacs.2021.100292 Final Published version
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Attachment(s)	Documents 82273456.pdf Final Published version, 9.24 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85112556455&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(a35c8e61-e449-47b8-b52d-030c280c2607).html

Abstract

We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm². Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.