Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Jiangbo Chen; Yachao Zhang; Songnan Bai; Jingyi Zhu; Pakpong Chirarattananon; Kai Ni; Qian Zhou; Lidai Wang

doi:10.1016/j.pacs.2021.100292

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Jiangbo Chen
, Yachao Zhang
, Songnan Bai
, Jingyi Zhu
, Pakpong Chirarattananon
, Kai Ni
, Qian Zhou
, Lidai Wang^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

22 Citations (Scopus)

70 Downloads (CityUHK Scholars)

Abstract

We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm². Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.

Original language	English
Article number	100292
Journal	Photoacoustics
Volume	23
Online published	9 Aug 2021
DOIs	https://doi.org/10.1016/j.pacs.2021.100292
Publication status	Published - Sept 2021

Research Keywords

Dual foci
Fast imaging
Fiber-based
OR-PAM
Resonant scanning

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This full text is made available under CC-BY-NC-ND 4.0. https://creativecommons.org/licenses/by-nc-nd/4.0/

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RGC-funded

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10.1016/j.pacs.2021.100292

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@article{a35c8e61e44947b8b52d030c280c2607,

title = "Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate",

abstract = "We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm2. Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.",

keywords = "Dual foci, Fast imaging, Fiber-based, OR-PAM, Resonant scanning",

author = "Jiangbo Chen and Yachao Zhang and Songnan Bai and Jingyi Zhu and Pakpong Chirarattananon and Kai Ni and Qian Zhou and Lidai Wang",

year = "2021",

month = sep,

doi = "10.1016/j.pacs.2021.100292",

language = "English",

volume = "23",

journal = "Photoacoustics",

issn = "2213-5979",

publisher = "Elsevier GmbH",

}

TY - JOUR

T1 - Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

AU - Chen, Jiangbo

AU - Zhang, Yachao

AU - Bai, Songnan

AU - Zhu, Jingyi

AU - Chirarattananon, Pakpong

AU - Ni, Kai

AU - Zhou, Qian

AU - Wang, Lidai

PY - 2021/9

Y1 - 2021/9

N2 - We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm2. Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.

AB - We report fiber-based dual-foci fast-scanning OR-PAM that can double the scanning rate without compromising the imaging resolution, the field of view, and the detection sensitivity. To achieve fast scanning speed, the OR-PAM system uses a single-axis water-immersible resonant scanning mirror that can confocally scan the optical and acoustic beams at 1018 Hz with a 3-mm range. Pulse energies of 45∼100-nJ are sufficient for acquiring vascular and oxygen-saturation images. The dual-foci method can double the B-scan rate to 2036 Hz. Using two lasers and stimulated Raman scattering, we achieve dual-wavelength excitation on both foci, and the total A-line rate is 3.2-MHz. In in vivo experiments, we inject epinephrine and monitor the hemodynamic and oxygen saturation response in the peripheral vessels at 1.7 Hz over 2.5 × 6.7 mm2. Dual-foci OR-PAM offers a new imaging tool for the study of fast physiological and pathological changes.

KW - Dual foci

KW - Fast imaging

KW - Fiber-based

KW - OR-PAM

KW - Resonant scanning

UR - https://www.scopus.com/pages/publications/85112556455

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85112556455&origin=recordpage

U2 - 10.1016/j.pacs.2021.100292

DO - 10.1016/j.pacs.2021.100292

M3 - RGC 21 - Publication in refereed journal

C2 - 34430201

SN - 2213-5979

VL - 23

JO - Photoacoustics

JF - Photoacoustics

M1 - 100292

ER -

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Abstract

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

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GRF: Development of Fast-scanning Hyperspectral Optical-resolution Photoacoustic Microscopy

GRF: Development of Optical-resolution Photoacoustic Microscopy with Compensation of Absorption Saturation

GRF: Quantitative Photoacoustic Imaging of Blood Oxygen Saturation in Deep Tissue

Cite this

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Abstract

Research Keywords

Publisher's Copyright Statement

RGC Funding Information

Access Output

Other Access Options

Permanent Link

Other Files and Links

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Projects

GRF: Development of Fast-scanning Hyperspectral Optical-resolution Photoacoustic Microscopy

GRF: Development of Optical-resolution Photoacoustic Microscopy with Compensation of Absorption Saturation

GRF: Quantitative Photoacoustic Imaging of Blood Oxygen Saturation in Deep Tissue

Cite this