Drug accumulation into single drug-sensitive and drug-resistant prostate cancer cells conducted on the single cell bioanalyzer

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

1 Scopus Citations
View graph of relations

Author(s)

  • Avid Khamenehfar
  • Ji Liu
  • Jia Cai
  • Michael Wong
  • Patrick Ling
  • Pamela Russell

Detail(s)

Original languageEnglish
Title of host publicationBiomedical and Biotechnology Engineering
PublisherAmerican Society of Mechanical Engineers (ASME)
Volume3
ISBN (Print)9780791846469
Publication statusPublished - 2014
Externally publishedYes

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE)
Volume3

Conference

TitleASME 2014 International Mechanical Engineering Congress and Exposition, IMECE 2014
PlaceCanada
CityMontreal
Period14 - 20 November 2014

Abstract

Multidrug resistance (MDR) occurs in prostate cancer, and this happens when the cancer cells resist chemotherapeutic drugs by pumping them out of the cells. MDR inhibitors such as cyclosporine A (CsA) can stop the pumping and enhance the drugs accumulated in the cells. The cellular drug accumulation is monitored using a microfluidic chip mounted on a single cell bioanalyzer. This equipment has been developed to measure accumulation of drugs such as doxorubicin (DOX) and fluorescently labeled paclitaxel (PTX) in single prostate cancer cells. The inhibition of drug efflux on the same prostate cell was examined in drugsensitive and drug-resistant cells. Accumulation of these drug molecules was not found in the MDR cells, PC-3 RX-DT2R cells. Enhanced drug accumulation was observed only after treating the MDR cell in the presence of 5 μM of CsA as the MDR inhibitor. We envision this monitoring of the accumulation of fluorescent molecules (drug or fluorescent molecules), if conducted on single patient cancer cells, can provide information for clinical monitoring of patients undergoing chemotherapy in the future.

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

Drug accumulation into single drug-sensitive and drug-resistant prostate cancer cells conducted on the single cell bioanalyzer. / Khamenehfar, Avid; Liu, Ji; Cai, Jia; Wong, Michael; Li, Paul C.H.; Ling, Patrick; Russell, Pamela.

Biomedical and Biotechnology Engineering. Vol. 3 American Society of Mechanical Engineers (ASME), 2014. (ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE); Vol. 3).

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review