Does a Hepadnavirus Contribute to Chronic Hepatitis or Hepatocellular Carcinoma in Dogs?
Research output: Conference Papers (RGC: 31A, 31B, 32, 33) › 32_Refereed conference paper (no ISBN/ISSN) › peer-review
Author(s)
Related Research Unit(s)
Detail(s)
| Original language | English |
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| Publication status | Published - Sep 2022 |
Conference
| Title | 6th International Society for Companion Animal Infectious Diseases Symposium (ISCAID 2022) |
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| Place | United Kingdom |
| City | Glasgow |
| Period | 4 - 7 September 2022 |
Link(s)
| Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(e9ee38f8-23e0-4e3f-ac7e-90c29bbb9a2b).html |
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Abstract
BACKGROUND
Hepatitis B virus (HBV), a hepadnavirus, causes chronic hepatitis (CH) and hepatocellular carcinoma (HCC) in humans. Domestic cat hepadnavirus (DCH) viraemia is detected in 3.2–12.3% of cats. In dogs, HBV DNA was detected in 10% of dog sera in Brazil; 6.3% of dog sera in Italy tested DCH qPCR positive. A hepadnavirus infecting dogs may play an aetiological role in canine idiopathic CH or HCC.
AIMS
To investigate whether hepadnaviral DNA is detected in a) blood from dogs in Hong Kong, and b) liver biopsies from dogs diagnosed with idiopathic CH or HCC.
METHODS
DCH qPCR was run on residual diagnostic canine blood DNAs. DNA extracted from formalin-fixed paraffin-embedded biopsies of idiopathic CH or HCC, diagnosed by board-certified pathologists at 3 institutions in Hong Kong and the USA, were tested using two panhepadnavirus cPCRs and one DCH-specific cPCR.
RESULTS
DCH DNA was amplified from two of 501 (0.4%) whole-blood derived DNA samples. DNA extracted from 101 liver biopsies (idiopathic CH n=47, HCC n=54) from dogs in Hong Kong and the USA, tested negative for DCH DNA, and also tested negative on both panhepadnavirus cPCRs.
DISCUSSION/ CONCLUSIONS
This study confirms that circulating DCH DNA can be detected in dogs in Hong Kong although rarely, in contrast to a recent investigation of dogs in Italy. No evidence to support a pathogenic role for a hepadnavirus in canine idiopathic CH or HCC was found.
Hepatitis B virus (HBV), a hepadnavirus, causes chronic hepatitis (CH) and hepatocellular carcinoma (HCC) in humans. Domestic cat hepadnavirus (DCH) viraemia is detected in 3.2–12.3% of cats. In dogs, HBV DNA was detected in 10% of dog sera in Brazil; 6.3% of dog sera in Italy tested DCH qPCR positive. A hepadnavirus infecting dogs may play an aetiological role in canine idiopathic CH or HCC.
AIMS
To investigate whether hepadnaviral DNA is detected in a) blood from dogs in Hong Kong, and b) liver biopsies from dogs diagnosed with idiopathic CH or HCC.
METHODS
DCH qPCR was run on residual diagnostic canine blood DNAs. DNA extracted from formalin-fixed paraffin-embedded biopsies of idiopathic CH or HCC, diagnosed by board-certified pathologists at 3 institutions in Hong Kong and the USA, were tested using two panhepadnavirus cPCRs and one DCH-specific cPCR.
RESULTS
DCH DNA was amplified from two of 501 (0.4%) whole-blood derived DNA samples. DNA extracted from 101 liver biopsies (idiopathic CH n=47, HCC n=54) from dogs in Hong Kong and the USA, tested negative for DCH DNA, and also tested negative on both panhepadnavirus cPCRs.
DISCUSSION/ CONCLUSIONS
This study confirms that circulating DCH DNA can be detected in dogs in Hong Kong although rarely, in contrast to a recent investigation of dogs in Italy. No evidence to support a pathogenic role for a hepadnavirus in canine idiopathic CH or HCC was found.
Citation Format(s)
Does a Hepadnavirus Contribute to Chronic Hepatitis or Hepatocellular Carcinoma in Dogs? / Choi, Yan Ru; Chen, Min-Chun; Carrai, Maura et al.
2022. Paper presented at 6th International Society for Companion Animal Infectious Diseases Symposium (ISCAID 2022), Glasgow, United Kingdom.Research output: Conference Papers (RGC: 31A, 31B, 32, 33) › 32_Refereed conference paper (no ISBN/ISSN) › peer-review
