DNA concentration on a microfabricated biochip intended for greenhouse-crop pathogen identification

M. Syrzycka; M. Sjoerdsma; D. Wicks; C. Koch; R. Utkhede; M. Syrzycki; P. Li; M. Parameswaran

doi:10.1109/CCECE.2002.1015264

DNA concentration on a microfabricated biochip intended for greenhouse-crop pathogen identification

M. Syrzycka
, M. Sjoerdsma
, D. Wicks
, C. Koch
, R. Utkhede
, M. Syrzycki
, P. Li
, M. Parameswaran

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

2 Citations (Scopus)

Abstract

Many plant diseases affecting greenhouse crops are caused by microbial pathogens, and their identification is a subjective, time-consuming and labor-intensive process. There is a critical need for a more rapid and accurate means of pathogen identification in agriculture since the methods to control diseases are more effective in the early infection stages. We propose to combine the microfabrication and MEMS technology as well as electronic DNA hybridization for accurate and rapid identification of plant pathogens. Our ultimate goal is to design, construct, and test a microfabricated biochip for accurate and rapid detection of two fungal plant pathogens, Didymella bryoniae and Botrytis cinerea, based on hybridization of pathogen DNA to immobilized DNA probes on glass substrates. This paper will discuss biochip design, fabrication, and assembly, as well as DNA probe concentration by electric field and optical detection. Experiments investigating electronic hybridization of pathogen DNA are underway.

Original language	English
Pages (from-to)	433-438
Journal	Canadian Conference on Electrical and Computer Engineering
Volume	1
DOIs	https://doi.org/10.1109/CCECE.2002.1015264
Publication status	Published - 2002
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Research Keywords

Biomedical Engineering
Nanotechnology and Micromachining

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10.1109/CCECE.2002.1015264

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title = "DNA concentration on a microfabricated biochip intended for greenhouse-crop pathogen identification",

abstract = "Many plant diseases affecting greenhouse crops are caused by microbial pathogens, and their identification is a subjective, time-consuming and labor-intensive process. There is a critical need for a more rapid and accurate means of pathogen identification in agriculture since the methods to control diseases are more effective in the early infection stages. We propose to combine the microfabrication and MEMS technology as well as electronic DNA hybridization for accurate and rapid identification of plant pathogens. Our ultimate goal is to design, construct, and test a microfabricated biochip for accurate and rapid detection of two fungal plant pathogens, Didymella bryoniae and Botrytis cinerea, based on hybridization of pathogen DNA to immobilized DNA probes on glass substrates. This paper will discuss biochip design, fabrication, and assembly, as well as DNA probe concentration by electric field and optical detection. Experiments investigating electronic hybridization of pathogen DNA are underway.",

keywords = "Biomedical Engineering, Nanotechnology and Micromachining, Biomedical Engineering, Nanotechnology and Micromachining, Biomedical Engineering, Nanotechnology and Micromachining",

author = "M. Syrzycka and M. Sjoerdsma and D. Wicks and C. Koch and R. Utkhede and M. Syrzycki and P. Li and M. Parameswaran",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].",

year = "2002",

doi = "10.1109/CCECE.2002.1015264",

language = "English",

volume = "1",

pages = "433--438",

journal = "Canadian Conference on Electrical and Computer Engineering",

issn = "0840-7789",

publisher = "IEEE",

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TY - JOUR

T1 - DNA concentration on a microfabricated biochip intended for greenhouse-crop pathogen identification

AU - Syrzycka, M.

AU - Sjoerdsma, M.

AU - Wicks, D.

AU - Koch, C.

AU - Utkhede, R.

AU - Syrzycki, M.

AU - Li, P.

AU - Parameswaran, M.

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2002

Y1 - 2002

N2 - Many plant diseases affecting greenhouse crops are caused by microbial pathogens, and their identification is a subjective, time-consuming and labor-intensive process. There is a critical need for a more rapid and accurate means of pathogen identification in agriculture since the methods to control diseases are more effective in the early infection stages. We propose to combine the microfabrication and MEMS technology as well as electronic DNA hybridization for accurate and rapid identification of plant pathogens. Our ultimate goal is to design, construct, and test a microfabricated biochip for accurate and rapid detection of two fungal plant pathogens, Didymella bryoniae and Botrytis cinerea, based on hybridization of pathogen DNA to immobilized DNA probes on glass substrates. This paper will discuss biochip design, fabrication, and assembly, as well as DNA probe concentration by electric field and optical detection. Experiments investigating electronic hybridization of pathogen DNA are underway.

AB - Many plant diseases affecting greenhouse crops are caused by microbial pathogens, and their identification is a subjective, time-consuming and labor-intensive process. There is a critical need for a more rapid and accurate means of pathogen identification in agriculture since the methods to control diseases are more effective in the early infection stages. We propose to combine the microfabrication and MEMS technology as well as electronic DNA hybridization for accurate and rapid identification of plant pathogens. Our ultimate goal is to design, construct, and test a microfabricated biochip for accurate and rapid detection of two fungal plant pathogens, Didymella bryoniae and Botrytis cinerea, based on hybridization of pathogen DNA to immobilized DNA probes on glass substrates. This paper will discuss biochip design, fabrication, and assembly, as well as DNA probe concentration by electric field and optical detection. Experiments investigating electronic hybridization of pathogen DNA are underway.

KW - Biomedical Engineering

KW - Nanotechnology and Micromachining

KW - Biomedical Engineering

KW - Nanotechnology and Micromachining

KW - Biomedical Engineering

KW - Nanotechnology and Micromachining

UR - https://www.scopus.com/pages/publications/0036064898

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-0036064898&origin=recordpage

U2 - 10.1109/CCECE.2002.1015264

DO - 10.1109/CCECE.2002.1015264

M3 - RGC 21 - Publication in refereed journal

SN - 0840-7789

VL - 1

SP - 433

EP - 438

JO - Canadian Conference on Electrical and Computer Engineering

JF - Canadian Conference on Electrical and Computer Engineering

ER -

DNA concentration on a microfabricated biochip intended for greenhouse-crop pathogen identification

Abstract

Bibliographical note

UN SDGs

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