DMD-based random-access optical-resolution photoacoustic microscopy

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

2 Scopus Citations
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Author(s)

  • Jinyang Liang
  • Yong Zhou
  • Amy W. Winkler
  • Konstantin I. Maslov
  • Chiye Li
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8943
ISBN (Print)9780819498564
Publication statusPublished - 2014
Externally publishedYes

Publication series

Name
Volume8943
ISSN (Print)1605-7422

Conference

TitlePhotons Plus Ultrasound: Imaging and Sensing 2014
PlaceUnited States
CitySan Francisco
Period2 - 5 February 2014

Abstract

The scanning mechanism is a major technical focus in optical-resolution photoacoustic microscopy. Flexible scanning access with fast scanning speed is desired to monitor biological and physiological dynamics with high temporal resolution. We developed random-access optical-resolution photoacoustic microscopy (RA-OR-PAM) using a digital micromirror device (DMD). Each micromirror on the DMD can be independently controlled, allowing imaging of regions of interest with arbitrary user-selected shapes without extraneous information. A global structural image is first acquired, and the regions of interest are selected. The laser beam then scans these regions exclusively, resulting in a faster frame rate than in a conventional raster scan. This system can rapidly scan arbitrarily shaped regions of interest with a lateral resolution of 3.6 μm within a 40×40 μm2 imaging area, a size comparable to the focal spot size of a 50 MHz ultrasound transducer. We demonstrated the random-access ability of RA-OR-PAM by imaging a monolayer of red blood cells. This system was then used to monitor blood flow in vivo within user-selected capillaries in a mouse ear. By imaging only the capillary of interest, the frame rate was increased by up to 13.3 times. © 2014 SPIE.

Research Area(s)

  • Medical and biological imaging, Photoacoustic imaging, Scanning microscopy

Citation Format(s)

DMD-based random-access optical-resolution photoacoustic microscopy. / Liang, Jinyang; Zhou, Yong; Winkler, Amy W. et al.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8943 SPIE, 2014. 894312.

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review