Dissolved organic matter with multi-peak fluorophores in landfill leachate

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Fan Lu
  • Cheng-Hsuan Chang
  • Pin-Jing He
  • Li-Ming Shao
  • Ay Su

Detail(s)

Original languageEnglish
Pages (from-to)575-582
Journal / PublicationChemosphere
Volume74
Issue number4
Publication statusPublished - Jan 2009
Externally publishedYes

Abstract

Dissolved organic matter (DOM) sampled from municipal landfill leachate of different ages with/without anoxic or aerobic treatment, was intensively fractionated via size exclusion chromatography (SEC) and hydrophobic resins, and was studied with fluorescence excitation and emission matrix (EEM). Six fluorophores with multiple EEM peaks (fluorophore A-F) were identified based on the collected EEM spectra and validated by bi-variate analysis, principal component analysis, and parallel factor analysis, as follows (excitation wavelength Ex and emission wavelength Em): (Ex 240, 310, 360 nm, Em 460 nm), (Ex 220, 280 nm, Em 340 nm), (Ex 220, 270 nm, Em 300 nm), (Ex 220, 280 nm, Em 360 nm), (Ex 230, 320 nm, Em 420 nm) and (Ex 220, 310 nm, Em 400 nm). The spectral characteristics of these fluorophores were discussed using fractional EEM and apparent molecular weight (AMW) data obtained via SEC analysis. The triple peak flurophore A was pointed at a hydrophobic acid or hydrophobic neutral compound with a pyrenyl functional group of AMW 2500-3500 Da, which displayed an excitation wavelength at 360 nm and a fluorescence intensity ratio of 6.70(±1.79):1.70(±0.41):1 (fluorescent intensities of Ex 240:Ex 310:Ex 360 nm at Ex 460 nm). This compound is observed to be refractory in landfilling or in anoxic/aerobic treatments, and is specific to this leachate contamination. This paper revealed that the coupling of SEC and EEM can be useful to track the fluorescent DOM fraction in landfill leachate. © 2008 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Excitation and emission matrix, Fluorophores, Landfill, Leachate, Size exclusion chromatography

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

Dissolved organic matter with multi-peak fluorophores in landfill leachate. / Lu, Fan; Chang, Cheng-Hsuan; Lee, Duu-Jong et al.
In: Chemosphere, Vol. 74, No. 4, 01.2009, p. 575-582.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review