Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska; Alessandra Torina; Vincenza Cannella; Santo Caracappa; Olivier A. E. Sparagano

doi:10.1007/s00436-004-1217-2

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Eva Spitalska
, Alessandra Torina
, Vincenza Cannella
, Santo Caracappa
, Olivier A. E. Sparagano

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

15 Citations (Scopus)

Abstract

Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

Original language	English
Pages (from-to)	318-320
Journal	Parasitology Research
Volume	94
Issue number	4
Online published	15 Sept 2004
DOIs	https://doi.org/10.1007/s00436-004-1217-2
Publication status	Published - Oct 2004
Externally published	Yes

Access Output

10.1007/s00436-004-1217-2

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

@article{d17abcfbb79347cc9840f549af719515,

title = "Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene",

abstract = "Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.",

author = "Eva Spitalska and Alessandra Torina and Vincenza Cannella and Santo Caracappa and Sparagano, \{Olivier A. E.\}",

year = "2004",

month = oct,

doi = "10.1007/s00436-004-1217-2",

language = "English",

volume = "94",

pages = "318--320",

journal = "Parasitology Research",

issn = "0932-0113",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "4",

}

TY - JOUR

T1 - Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

AU - Spitalska, Eva

AU - Torina, Alessandra

AU - Cannella, Vincenza

AU - Caracappa, Santo

AU - Sparagano, Olivier A. E.

PY - 2004/10

Y1 - 2004/10

N2 - Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

AB - Theileria lestoquardi and T. annulata can occur in similar vectors, and current available probes based on the 18S rRNA gene showed cross-reaction between the two species. However, we developed a species-specific RFLP test based on the MspI restriction enzyme, able to cut amplified products from T. lestoquardi only and to discriminate the two species in both tick and blood samples.

UR - https://www.scopus.com/pages/publications/8744251033

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-8744251033&origin=recordpage

U2 - 10.1007/s00436-004-1217-2

DO - 10.1007/s00436-004-1217-2

M3 - RGC 21 - Publication in refereed journal

C2 - 15372232

SN - 0932-0113

VL - 94

SP - 318

EP - 320

JO - Parasitology Research

JF - Parasitology Research

IS - 4

ER -

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

Abstract

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this