Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

8 Scopus Citations
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Author(s)

  • Kingsley King-Gee Tam
  • Kenneth Siu-Sing Leung
  • Sabrina Wai-Chi To
  • Gilman Kit-Hang Siu
  • Victor Chi-Man Shek
  • Cindy Wing-Sze Tse
  • Samson Sai-Yin Wong
  • Pak-Leung Ho
  • Wing-Cheong Yam

Related Research Unit(s)

Detail(s)

Original languageEnglish
Pages (from-to)118-124
Journal / PublicationDiagnostic Microbiology and Infectious Disease
Volume89
Issue number2
Online published1 Jul 2017
Publication statusPublished - Oct 2017

Abstract

Abbott RealTime MTB (Abbott-RT) in conjunction with Abbott RealTime MTB RIF/INH Resistance (Abbott-RIF/INH) is a new, high-throughput automated nucleic acid amplification platform (Abbott-MDR) for detection of Mycobacterium tuberculosis complex (MTBC) and the genotypic markers for rifampicin (RIF) and isoniazid (INH) resistance directly from respiratory specimens. This prospective study evaluated the diagnostic performance of this new platform for MTBC and multidrug-resistant tuberculosis (MDR-TB) using 610 sputum specimens in a tuberculosis high-burden setting. Using conventional culture results and clinical background as reference standards, Abbott-RT exhibited an overall sensitivity and specificity of 95.2% and 99.8%, respectively. Genotypic RIF/INH resistance of 178 “MTB detected” specimens was subsequently analyzed by Abbott-RIF/INH. Compared to phenotypic drug susceptibility test results, Abbott-RIF/INH detected resistance genotypic markers in 84.6% MDR-TB, 80% mono-RIF-resistant and 66.7% mono-INH-resistant specimens. Two of the RIF-resistant specimens carried a novel single, nonsense mutation at rpoB  Q513 and in silico simulation demonstrated that the truncated RpoB protein failed to bind with other subunits for transcription. Overall, Abbott-MDR platform provided high throughput and reliable diagnosis of MDR-TB within a TB high-burden region.

Research Area(s)

  • Direct detection, Multidrug-resistant tuberculosis, Mycobacteriology, Mycobacterium tuberculosis, Respiratory specimen

Citation Format(s)

Direct detection of Mycobacterium tuberculosis and drug resistance in respiratory specimen using Abbott Realtime MTB detection and RIF/INH resistance assay. / Tam, Kingsley King-Gee; Leung, Kenneth Siu-Sing; To, Sabrina Wai-Chi; Siu, Gilman Kit-Hang; Lau, Terrence Chi-Kong; Shek, Victor Chi-Man; Tse, Cindy Wing-Sze; Wong, Samson Sai-Yin; Ho, Pak-Leung; Yam, Wing-Cheong.

In: Diagnostic Microbiology and Infectious Disease, Vol. 89, No. 2, 10.2017, p. 118-124.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal