Abstract
Cryopreservation is a key technology in biology and clinical practice. This paper presents a digital microfluidic device that automates sample preparation for mammalian embryo vitrification. Individual micro droplets manipulated on the microfluidic device were used as micro-vessels to transport a single mouse embryo through a complete vitrification procedure. Advantages of this approach, compared to manual operation and channel-based microfluidic vitrification, include automated operation, cryoprotectant concentration gradient generation, and feasibility of loading and retrieval of embryos.
| Original language | English |
|---|---|
| Article number | e108128 |
| Journal | PLoS ONE |
| Volume | 9 |
| Issue number | 9 |
| Online published | 24 Sept 2014 |
| DOIs | |
| Publication status | Published - Sept 2014 |
| Externally published | Yes |
Publisher's Copyright Statement
- This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
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