Differently Environment Stable Bio-Silver Nanoparticles : Study on Their Optical Enhancing and Antibacterial Properties

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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  • Shanmugam Girija
  • Rajendran Selvakumar
  • Saowanit Tongpim
  • Arno C. Gutleb
  • Sarvajeyakesavalu Suriyanarayanan


Original languageEnglish
Article numbere77043
Journal / PublicationPLOS ONE
Issue number10
Publication statusPublished - 9 Oct 2013
Externally publishedYes



Generally, limited research is extended in studying stability and applicational properties of silver nanoparticles (Ag NPs) synthesized by adopting 'green chemistry' protocol. In this work, we report on the synthesis of stable Ag NPs using plant-derived materials such as leaf extract of Neem (Azadirachta indica) and biopolymer pectin from apple peel. In addition, the applicational properties of Ag NPs such as surface-enhanced Raman scattering (SERS) and antibacterial efficiencies were also investigated. As-synthesized nanoparticles (NPs) were characterized using various instrumentation techniques. Both the plant materials (leaf extract and biopolymer) favored the synthesis of well-defined NPs capped with biomaterials. The NPs were spherical in shape with an average particle size between 14-27 nm. These bio-NPs exhibited colloidal stability in most of the suspended solutions such as water, electrolyte solutions (NaCl; NaNO3), biological solution (bovine serum albumin), and in different pH solutions (pH 7; 9) for a reasonable time period of 120 hrs. Both the bio-NPs were observed to be SERS active through displaying intrinsic SERS signals of the Raman probe molecule (Nile blue A). The NPs were effective against the Escherichia coli bacterium when tested in nutrient broth and agar medium. Scanning and high-resolution transmission electron microscopy (SEM and HRTEM) images confirmed cellular membrane damage of nanoparticle treated E. coli cells. These environmental friendly template Ag NPs can be used as an antimicrobial agent and also for SERS based analytical applications. © 2013 Balachandran et al.

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Differently Environment Stable Bio-Silver Nanoparticles: Study on Their Optical Enhancing and Antibacterial Properties. / Balachandran, Yekkuni L.; Girija, Shanmugam; Selvakumar, Rajendran et al.
In: PLOS ONE, Vol. 8, No. 10, e77043, 09.10.2013.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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