Differentially-Charged Liposomes Interact with Alphaherpesviruses and Interfere with Virus Entry

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

1 Scopus Citations
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Author(s)

  • Oleksandr Kolyvushko
  • Juliane Latzke
  • Ismail Dahmani
  • Salvatore Chiantia
  • Walid Azab

Detail(s)

Original languageEnglish
Article number359
Journal / PublicationPathogens
Volume9
Issue number5
Online published8 May 2020
Publication statusPublished - May 2020
Externally publishedYes

Abstract

Exposure of phosphatidylserine (PS) in the outer leaflet of the plasma membrane is induced by infection with several members of the Alphaherpesvirinae subfamily. There is evidence that PS is used by the equine herpesvirus type 1 (EHV-1) during entry, but the exact role of PS and other phospholipids in the entry process remains unknown. Here, we investigated the interaction of differently charged phospholipids with virus particles and determined their influence on infection. Our data show that liposomes containing negatively charged PS or positively charged DOTAP (N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium) inhibited EHV-1 infection, while neutral phosphatidylcholine (PC) had no effect. Inhibition of infection with PS was transient, decreased with time, and was dose dependent. Our findings indicate that both cationic and anionic phospholipids can interact with the virus and reduce infectivity, while, presumably, acting through different mechanisms. Charged phospholipids were found to have antiviral effects and may be used to inhibit EHV-1 infection.

Research Area(s)

  • Alphaherpesvirus, EHV-1, Inhibition, Pathogen host interaction, Phosphatidylserine

Citation Format(s)

Differentially-Charged Liposomes Interact with Alphaherpesviruses and Interfere with Virus Entry. / Kolyvushko, Oleksandr; Latzke, Juliane; Dahmani, Ismail; Osterrieder, Nikolaus; Chiantia, Salvatore; Azab, Walid.

In: Pathogens, Vol. 9, No. 5, 359, 05.2020.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review