Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells

Jin Wang; Fang-Ting He; Chi-Hung Tzang; Wang-Fan Fong; Pei-Gen Xiao; Rui Han; Meng-Su Yang

doi:10.16438/j.0513-4870.2005.12.009

Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells

Jin Wang, Fang-Ting He, Chi-Hung Tzang, Wang-Fan Fong, Pei-Gen Xiao, Rui Han, Meng-Su Yang^*

^*Corresponding author for this work

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

10 Citations (Scopus)

Abstract

Aim: To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. Methods: Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9 984 genes and expressed sequence tags (ESTs). Results: Cell cycle analysis showed that 77.8% of cells arrested at G₂/M phase and 1.3% of cells underwent apoptosis upon 100 nmol · L^-1 paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol · L^-1 (IC₅₀) and 100 nmol · L^-1 paclitaxel treatment, respectively. Conclusion: Paclitaxel stabilized microtubules and caused G₂/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.

Translated title of the contribution	紫杉醇诱导人乳腺癌 MCF-7 细胞周期阻断及凋亡的基因表达谱分析
Original language	English
Pages (from-to)	1099-1104
Journal	Acta Pharmaceutica Sinica
Volume	40
Issue number	12
DOIs	https://doi.org/10.16438/j.0513-4870.2005.12.009
Publication status	Published - Dec 2005

Research Keywords

cDNA microarray
Paclitaxel
Gene expression
MCF-7 cells
Apoptosis
基因芯片
紫杉醇
基因表达
MCF-7 细胞
细胞凋亡

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title = "Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells",

abstract = "Aim: To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. Methods: Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9 984 genes and expressed sequence tags (ESTs). Results: Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol · L-1 paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol · L-1 (IC50) and 100 nmol · L-1 paclitaxel treatment, respectively. Conclusion: Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.",

keywords = "cDNA microarray, Paclitaxel, Gene expression, MCF-7 cells, Apoptosis, 基因芯片, 紫杉醇, 基因表达, MCF-7 细胞, 细胞凋亡, 基因芯片, 紫杉醇, 基因表達, MCF-7 細胞, 細胞凋亡",

author = "Jin Wang and Fang-Ting He and Chi-Hung Tzang and Wang-Fan Fong and Pei-Gen Xiao and Rui Han and Meng-Su Yang",

year = "2005",

month = dec,

doi = "10.16438/j.0513-4870.2005.12.009",

language = "English",

volume = "40",

pages = "1099--1104",

journal = "Acta Pharmaceutica Sinica",

issn = "0513-4870",

publisher = "Chinese Pharmaceutical Association",

number = "12",

}

TY - JOUR

T1 - Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells

AU - Wang, Jin

AU - He, Fang-Ting

AU - Tzang, Chi-Hung

AU - Fong, Wang-Fan

AU - Xiao, Pei-Gen

AU - Han, Rui

AU - Yang, Meng-Su

PY - 2005/12

Y1 - 2005/12

N2 - Aim: To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. Methods: Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9 984 genes and expressed sequence tags (ESTs). Results: Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol · L-1 paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol · L-1 (IC50) and 100 nmol · L-1 paclitaxel treatment, respectively. Conclusion: Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.

AB - Aim: To elucidate the molecular mechanism of cell cycle arrest and apoptosis of MCF-7 cells induced by paclitaxel. Methods: Flow cytometry was used to determine the cell cycle changes of MCF-7 cells upon paclitaxel treatment. Gene expression profiles of MCF-7 cells induced by paclitaxel were obtained by using cDNA microarrays containing 9 984 genes and expressed sequence tags (ESTs). Results: Cell cycle analysis showed that 77.8% of cells arrested at G2/M phase and 1.3% of cells underwent apoptosis upon 100 nmol · L-1 paclitaxel treatment for 24 hours; cDNA microarray results revealed that 27 and 77 genes were differentially expressed upon 12.5 nmol · L-1 (IC50) and 100 nmol · L-1 paclitaxel treatment, respectively. Conclusion: Paclitaxel stabilized microtubules and caused G2/M cell cycle arrest and apoptotic cell death in a concentration-dependent manner, which is associated with the regulation of selected genes related to microtubule assembly and cytoskeleton, cell cycle regulation, and DNA repair and apoptosis.

KW - cDNA microarray

KW - Paclitaxel

KW - Gene expression

KW - MCF-7 cells

KW - Apoptosis

KW - 基因芯片

KW - 紫杉醇

KW - 基因表达

KW - MCF-7 细胞

KW - 细胞凋亡

KW - 基因芯片

KW - 紫杉醇

KW - 基因表達

KW - MCF-7 細胞

KW - 細胞凋亡

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DO - 10.16438/j.0513-4870.2005.12.009

M3 - RGC 21 - Publication in refereed journal

C2 - 16496673

SN - 0513-4870

VL - 40

SP - 1099

EP - 1104

JO - Acta Pharmaceutica Sinica

JF - Acta Pharmaceutica Sinica

IS - 12

ER -

Differential gene expression profiles in paclitaxel-induced cell cycle arrest and apoptosis in human breast cancer MCF-7 cells

Abstract

Research Keywords

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