TY - GEN
T1 - Different optical images for optically-induced electroporation of multiple gene transfection
AU - Lee, You-Hsun
AU - Wang, Chih-Hung
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2013
Y1 - 2013
N2 - Gene transfection plays an important role in biological researches, especially in the development of induced pluripotent stem cells (iPS). In this work, an optically-induced electroporation method for gene transfection by using a microfluidic platform was presented. Electroporation can be induced by illuminating an optical image on a photoconductive layer when the top and the bottom substrates were applied with an electric field. One of the most important factors that affected efficiency of optically-induced gene transfection was the optical patterns illuminating on the chip. Therefore, a series of various optical patterns, including multiple circles, squares, and triangles, were tested in order to enhance the efficiency of gene transfection. Consequently, the pattern of multiple triangular spots was experimentally confirmed to have the highest efficiency of gene transfection. In addition, three fluorescence carried plasmids could be successfully transfected into different types of cells by using the developed optically-induced electroporation platform at the same time. The developed microfluidic system may be promising for iPS research. © 2013 IEEE.
AB - Gene transfection plays an important role in biological researches, especially in the development of induced pluripotent stem cells (iPS). In this work, an optically-induced electroporation method for gene transfection by using a microfluidic platform was presented. Electroporation can be induced by illuminating an optical image on a photoconductive layer when the top and the bottom substrates were applied with an electric field. One of the most important factors that affected efficiency of optically-induced gene transfection was the optical patterns illuminating on the chip. Therefore, a series of various optical patterns, including multiple circles, squares, and triangles, were tested in order to enhance the efficiency of gene transfection. Consequently, the pattern of multiple triangular spots was experimentally confirmed to have the highest efficiency of gene transfection. In addition, three fluorescence carried plasmids could be successfully transfected into different types of cells by using the developed optically-induced electroporation platform at the same time. The developed microfluidic system may be promising for iPS research. © 2013 IEEE.
UR - https://www.scopus.com/pages/publications/84875467538
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84875467538&origin=recordpage
U2 - 10.1109/MEMSYS.2013.6474391
DO - 10.1109/MEMSYS.2013.6474391
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781467356558
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 907
EP - 910
BT - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
T2 - IEEE 26th International Conference on Micro Electro Mechanical Systems, MEMS 2013
Y2 - 20 January 2013 through 24 January 2013
ER -