Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chih-Hung Wang
  • You-Hsun Lee
  • Hsin-Tzu Kuo
  • Wen-Feng Liang
  • Gwo-Bin Lee

Detail(s)

Original languageEnglish
Pages (from-to)592-601
Journal / PublicationLab on a Chip - Miniaturisation for Chemistry and Biology
Volume14
Issue number3
Online published8 Nov 2013
Publication statusPublished - 7 Feb 2014

Abstract

Gene transfection is an important technology for various biological applications. The exogenous DNA is commonly delivered into cells by using a strong electrical field to form transient pores in cellular membranes. However, the high voltage required in this electroporation process may cause cell damage. In this study, a dielectrophoretically-assisted electroporation was developed by using light-activated virtual microelectrodes in a new microfluidic platform. The DNA electrotransfection used a low applied voltage and an alternating current to enable electroporation and transfection. Single or triple fluorescence-carrying plasmids were effectively transfected into various types of mammalian cells, and the fluorescent proteins were successfully expressed in live transfected cells. Moreover, the multi-triangle optical pattern that was projected onto a photoconductive layer to generate localized non-uniform virtual electric fields was found to have high transfection efficiency. The developed dielectrophoretically-assisted electroporation platform may provide a simpler system for gene transfection and could be widely applied in many biotechnological fields. © 2014 The Royal Society of Chemistry.

Citation Format(s)

Dielectrophoretically-assisted electroporation using light-activated virtual microelectrodes for multiple DNA transfection. / Wang, Chih-Hung; Lee, You-Hsun; Kuo, Hsin-Tzu et al.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 14, No. 3, 07.02.2014, p. 592-601.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review