Dielectrophoretic trapping of single leukemic cells using the conventional and compact optical measurement systems

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Hamideh Sharifi Noghabi
  • Mandy Soo
  • Avid Khamenehfar
  • Paul C.H. Li

Detail(s)

Original languageEnglish
Pages (from-to)1478-1485
Journal / PublicationElectrophoresis
Volume40
Issue number10
Publication statusPublished - 1 May 2019
Externally publishedYes

Abstract

Here, we report a microfluidic same-single-cell analysis to study the inhibition of multidrug resistance due to drug efflux on single leukemic cells. Drug efflux inhibition was investigated in the microfluidic chip using two different fluorescence detection systems, namely, a compact single-cell bioanalyzer and the conventional optical detection system constructed from an inverted microscope and a microphotometer. More importantly, a compact signal generator was used to conduct dielectrophoretic cell trapping together with the compact SCB. By using the DEP force, a single acute myeloid leukemia cell was trapped in the cell retention structure of the chip. This allowed us to detect dye accumulation in the MDR leukemic cells in the presence of cyclosporine A (CsA). CsA and rhodamine 123 were used as the P-glycoprotein inhibitor and fluorescent dye, respectively. The result showed that the Rh123 fluorescence signal in a single-cell increased dramatically over its same-cell control on both fluorescence detection systems due to the inhibition by CsA.

Research Area(s)

  • Acute myeloid leukemic cell, Compact fluorescence measurement, Dielectrophoretic force, Microfluidic cell retention, Multidrug resistance, Single-cell analysis

Bibliographic Note

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Citation Format(s)

Dielectrophoretic trapping of single leukemic cells using the conventional and compact optical measurement systems. / Sharifi Noghabi, Hamideh; Soo, Mandy; Khamenehfar, Avid; Li, Paul C.H.

In: Electrophoresis, Vol. 40, No. 10, 01.05.2019, p. 1478-1485.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review