Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity

Chi-Chun Fong; Yaou Zhang; Qi Zhang; Chi-Hung Tzang; Wang-Fun Fong; Rodulf S.S. Wu; Mengsu Yang

doi:10.1016/j.tox.2007.03.024

Author(s)

Chi-Chun Fong
Yaou Zhang
Qi Zhang
Chi-Hung Tzang
Wang-Fun Fong
Rodulf S.S. Wu

Detail(s)

Original language	English
Pages (from-to)	16-28
Journal / Publication	Toxicology
Volume	236
Issue number	1-2
Publication status	Published - 1 Jul 2007

Link(s)

DOI	DOI https://doi.org/10.1016/j.tox.2007.03.024 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-34248639057&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(f61e3e80-73b0-4f7c-91e5-192744a07b98).html

Abstract

In this study, the effect of dexamethasone, a synthetic glucocorticoid, on H₂O₂ stimulated murine RAW264.7 macrophages was investigated. It was found that dexamethasone protected the cells from apoptosis induced by H₂O₂. A cDNA microarray, which consists of 1000 genes selected from a mouse clone set provided from NIA, was used to study the gene expression profiles involved in the protective effect. Our data show that dexamethasone exerts the anti-apoptosis function by changing the expression patterns of many genes involved inhibiting the up-regulation of apoptosis promoting genes and the down-regulation of cell cycle stimulating genes as well as keeping the up-regulation of cell survival related genes. Our study also revealed that dexamethasone protects RAW264.7 macrophages from H₂O₂ induced apoptosis through blocking nuclear factor-kappa B (NF-κB) activity. © 2007 Elsevier Ireland Ltd. All rights reserved.

Research Area(s)

Dexamethasone, Gene expression, Hydrogen peroxide, NF-κB activity, RAW264.7 macrophages

Citation Format(s)

[ RIS ] [ BibTeX ]

Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity. / Fong, Chi-Chun; Zhang, Yaou; Zhang, Qi et al.
In: Toxicology, Vol. 236, No. 1-2, 01.07.2007, p. 16-28.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Fong, C-C, Zhang, Y, Zhang, Q, Tzang, C-H, Fong, W-F, Wu, RSS & Yang, M 2007, 'Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity', Toxicology, vol. 236, no. 1-2, pp. 16-28. https://doi.org/10.1016/j.tox.2007.03.024

Fong, C.-C., Zhang, Y., Zhang, Q., Tzang, C.-H., Fong, W.-F., Wu, R. S. S., & Yang, M. (2007). Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity. Toxicology, 236(1-2), 16-28. https://doi.org/10.1016/j.tox.2007.03.024

Fong CC, Zhang Y, Zhang Q, Tzang CH, Fong WF, Wu RSS et al. Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity. Toxicology. 2007 Jul 1;236(1-2):16-28. doi: 10.1016/j.tox.2007.03.024

Fong, Chi-Chun ; Zhang, Yaou ; Zhang, Qi et al. / Dexamethasone protects RAW264.7 macrophages from growth arrest and apoptosis induced by H2O2 through alteration of gene expression patterns and inhibition of nuclear factor-kappa B (NF-κB) activity. In: Toxicology. 2007 ; Vol. 236, No. 1-2. pp. 16-28.