Abstract
A novel approach based on liquid marble is demonstrated to realize in situ tumor spheroid culture, which can be used to understand how tumor spheroids response to external environment in three dimensions. The sizes of coating particles impact the transmissivity of liquid marble and liquid marble coated by silica nanoparticle showed high transparency then could substitute for cuvette in absorbance measurement. Furthermore, culture process of tumor spheroids from bulk cells or single cell in liquid marble could be recorded clearly due to high transparence of liquid marble. Tumor spheroids cultured in liquid marbles were placed in 384-well plate or liquid marbles, and the viability change of spheroids in continuous culture under simulated hypoxic conditions revealed that liquid marbles are gas-sensitive. This platform could be applied in analyzing the effect of anti-cancer drugs and anticancer siRNAs (small interfering RNA) at different concentration on tumor spheroids, which indicated that the liquid marble had the feasibility to work as a bioreactor for drug screening.
| Original language | English |
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| Publication status | Presented - 22 Sept 2016 |
| Event | Cancer Stem Cell Conference 2016 - Cleveland, Ohio, Cleveland, United States Duration: 20 Sept 2016 → 23 Sept 2016 https://case.edu/medicine/ncrm/csc/ |
Conference
| Conference | Cancer Stem Cell Conference 2016 |
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| Abbreviated title | CSC 2016 |
| Place | United States |
| City | Cleveland |
| Period | 20/09/16 → 23/09/16 |
| Internet address |