Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study

Umberto Molini; Narut Thanantong; Annunziata Giangaspero; Olivier A. E. Sparagano

doi:10.1196/annals.1428.049

Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study

Umberto Molini
, Narut Thanantong
, Annunziata Giangaspero
, Olivier A. E. Sparagano^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

4 Citations (Scopus)

Abstract

Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

Original language	English
Title of host publication	Animal Biodiversity and Emerging Diseases
Subtitle of host publication	Prediction and Prevention
Editors	Olivier A.E. Sparagano, Jean-Charles Maillard, Julio Vicente Figueroa
Publisher	Wiley-Blackwell
Pages	312-314
ISBN (Print)	9781573317146
DOIs	https://doi.org/10.1196/annals.1428.049
Publication status	Published - Dec 2008
Externally published	Yes
Event	9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007) - Merida, Mexico Duration: 17 Jun 2007 → 22 Jun 2007

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1149
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Conference

Conference	9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007)
Abbreviated title	STVM 2007
Place	Mexico
City	Merida
Period	17/06/07 → 22/06/07

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Research Keywords

Diagnostic
PCR
Reverse line blot
Vibrio

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Molini, U., Thanantong, N., Giangaspero, A., & Sparagano, O. A. E. (2008). Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study. In O. A. E. Sparagano, J.-C. Maillard, & J. V. Figueroa (Eds.), Animal Biodiversity and Emerging Diseases: Prediction and Prevention (pp. 312-314). (Annals of the New York Academy of Sciences; Vol. 1149). Wiley-Blackwell. https://doi.org/10.1196/annals.1428.049

Molini, Umberto ; Thanantong, Narut ; Giangaspero, Annunziata et al. / Development of Specific Oligonucleotide Probes to Detect Vibrio Species : A Preliminary Study. Animal Biodiversity and Emerging Diseases: Prediction and Prevention. editor / Olivier A.E. Sparagano ; Jean-Charles Maillard ; Julio Vicente Figueroa. Wiley-Blackwell, 2008. pp. 312-314 (Annals of the New York Academy of Sciences).

@inproceedings{69ed07cec6fc43eab5fe6ff21331bfc2,

title = "Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study",

abstract = "Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks. ",

keywords = "Diagnostic, PCR, Reverse line blot, Vibrio",

author = "Umberto Molini and Narut Thanantong and Annunziata Giangaspero and Sparagano, \{Olivier A. E.\}",

year = "2008",

month = dec,

doi = "10.1196/annals.1428.049",

language = "English",

isbn = "9781573317146",

series = "Annals of the New York Academy of Sciences",

publisher = "Wiley-Blackwell",

pages = "312--314",

editor = "Sparagano, \{Olivier A.E.\} and Jean-Charles Maillard and Figueroa, \{Julio Vicente\}",

booktitle = "Animal Biodiversity and Emerging Diseases",

address = "United States",

note = "9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007), STVM 2007 ; Conference date: 17-06-2007 Through 22-06-2007",

}

Molini, U, Thanantong, N, Giangaspero, A & Sparagano, OAE 2008, Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study. in OAE Sparagano, J-C Maillard & JV Figueroa (eds), Animal Biodiversity and Emerging Diseases: Prediction and Prevention. Annals of the New York Academy of Sciences, vol. 1149, Wiley-Blackwell, pp. 312-314, 9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007), Merida, Mexico, 17/06/07. https://doi.org/10.1196/annals.1428.049

Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study. / Molini, Umberto; Thanantong, Narut; Giangaspero, Annunziata et al.
Animal Biodiversity and Emerging Diseases: Prediction and Prevention. ed. / Olivier A.E. Sparagano; Jean-Charles Maillard; Julio Vicente Figueroa. Wiley-Blackwell, 2008. p. 312-314 (Annals of the New York Academy of Sciences; Vol. 1149).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Development of Specific Oligonucleotide Probes to Detect Vibrio Species

T2 - 9th Biennial Conference of the International Society for Tropical Veterinary Medicine (STVM 2007)

AU - Molini, Umberto

AU - Thanantong, Narut

AU - Giangaspero, Annunziata

AU - Sparagano, Olivier A. E.

PY - 2008/12

Y1 - 2008/12

N2 - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

AB - Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V. parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

KW - Diagnostic

KW - PCR

KW - Reverse line blot

KW - Vibrio

UR - https://www.scopus.com/pages/publications/57849098915

UR - https://www.scopus.com/pages/publications/57849098915#tab=impact

U2 - 10.1196/annals.1428.049

DO - 10.1196/annals.1428.049

M3 - RGC 32 - Refereed conference paper (with host publication)

C2 - 19120237

SN - 9781573317146

T3 - Annals of the New York Academy of Sciences

SP - 312

EP - 314

BT - Animal Biodiversity and Emerging Diseases

A2 - Sparagano, Olivier A.E.

A2 - Maillard, Jean-Charles

A2 - Figueroa, Julio Vicente

PB - Wiley-Blackwell

Y2 - 17 June 2007 through 22 June 2007

ER -

Molini U, Thanantong N, Giangaspero A, Sparagano OAE. Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study. In Sparagano OAE, Maillard JC, Figueroa JV, editors, Animal Biodiversity and Emerging Diseases: Prediction and Prevention. Wiley-Blackwell. 2008. p. 312-314. (Annals of the New York Academy of Sciences). Epub 2008 Dec 15. doi: 10.1196/annals.1428.049

Development of Specific Oligonucleotide Probes to Detect Vibrio Species: A Preliminary Study

Abstract

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UN SDGs

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